NDST1

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 is an enzyme. In humans, it is encoded by the NDST1 gene.[5][6][7]

NDST1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1NST
Identifiers
Aliases	NDST1, HSST, NST1, MRT46, N-deacetylase/N-sulfotransferase 1, N-deacetylase and N-sulfotransferase 1
External IDs	OMIM: 600853 MGI: 104719 HomoloGene: 20386 GeneCards: NDST1
Gene location (Human) Chr. Chromosome 5 (human)[1] Band 5q33.1 Start 150,485,818 bp[1] End 150,558,211 bp[1]
Gene location (Mouse) Chr. Chromosome 18 (mouse)[2] Band 18|18 D3 Start 60,684,494 bp[2] End 60,748,650 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • sulfotransferase activity • deacetylase activity • catalytic activity • hydrolase activity • N-acetylglucosamine deacetylase activity • GO:0001948 protein binding • [heparan sulfate-glucosamine N-sulfotransferase activity] • heparan sulfate N-acetylglucosaminyltransferase activity • heparan sulfate sulfotransferase activity Cellular component • integral component of membrane • Golgi apparatus • membrane • Golgi membrane Biological process • cardiac septum development • heparin biosynthetic process • embryonic neurocranium morphogenesis • coronary vasculature development • respiratory gaseous exchange • fibroblast growth factor receptor signaling pathway • aorta development • heart development • glycosaminoglycan biosynthetic process • animal organ morphogenesis • embryonic viscerocranium morphogenesis • polysaccharide biosynthetic process • forebrain development • metabolism • protein sulfation • inflammatory response • midbrain development • glycosaminoglycan metabolic process • heparan sulfate proteoglycan biosynthetic process • heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process • positive regulation of MAPK cascade • positive regulation of smoothened signaling pathway • sulfur compound metabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	3340	15531
Ensembl	ENSG00000070614	ENSMUSG00000054008
UniProt	P52848	Q3UHN9
RefSeq (mRNA)	NM_001301063 NM_001543	NM_008306 NM_001348100 NM_001348101 NM_001348102 NM_001348103 NM_001348146
RefSeq (protein)	NP_001287992 NP_001534	NP_032332 NP_001335029 NP_001335030 NP_001335031 NP_001335032 NP_001335075
Location (UCSC)	Chr 5: 150.49 – 150.56 Mb	Chr 18: 60.68 – 60.75 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000070614 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000054008 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Dixon J, Loftus SK, Gladwin AJ, Scambler PJ, Wasmuth JJ, Dixon MJ (Aug 1995). "Cloning of the human heparan sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher Collins syndrome candidate region at 5q32-q33.1". Genomics. 26 (2): 239–44. doi:10.1016/0888-7543(95)80206-2. PMID 7601448.
6. Humphries DE, Sullivan BM, Aleixo MD, Stow JL (Aug 1997). "Localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase to the trans-Golgi network". Biochem J. 325. ( Pt 2) (2): 351–7. doi:10.1042/bj3250351. PMC 1218567. PMID 9230113.
7. "Entrez Gene: NDST1 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1".

Further reading

• Sueyoshi T, Kakuta Y, Pedersen LC, et al. (1998). "A role of Lys614 in the sulfotransferase activity of human heparan sulfate N-deacetylase/N-sulfotransferase". FEBS Lett. 433 (3): 211–4. doi:10.1016/S0014-5793(98)00913-2. PMID 9744796.
• Aikawa J, Esko JD (1999). "Molecular cloning and expression of a third member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family". J. Biol. Chem. 274 (5): 2690–5. doi:10.1074/jbc.274.5.2690. PMID 9915799.
• Kakuta Y, Sueyoshi T, Negishi M, Pedersen LC (1999). "Crystal structure of the sulfotransferase domain of human heparan sulfate N-deacetylase/ N-sulfotransferase 1". J. Biol. Chem. 274 (16): 10673–6. doi:10.1074/jbc.274.16.10673. PMID 10196134.
• Pikas DS, Eriksson I, Kjellén L (2000). "Overexpression of different isoforms of glucosaminyl N-deacetylase/N-sulfotransferase results in distinct heparan sulfate N-sulfation patterns". Biochemistry. 39 (15): 4552–8. doi:10.1021/bi992524l. PMID 10758005.
• Mura CV, Becker MI, Orellana A, Wolff D (2002). "Immunopurification of Golgi vesicles by magnetic sorting". J. Immunol. Methods. 260 (1–2): 263–71. doi:10.1016/S0022-1759(01)00546-4. PMID 11792394.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Bengtsson J, Eriksson I, Kjellén L (2003). "Distinct effects on heparan sulfate structure by different active site mutations in NDST-1". Biochemistry. 42 (7): 2110–5. doi:10.1021/bi026928g. PMID 12590599.
• van den Born J, Pikas DS, Pisa BJ, et al. (2003). "Antibody-based assay for N-deacetylase activity of heparan sulfate/heparin N-deacetylase/N-sulfotransferase (NDST): novel characteristics of NDST-1 and -2". Glycobiology. 13 (1): 1–10. doi:10.1093/glycob/cwg011. PMID 12634318.
• Nakayama K, Natori Y, Sato T, et al. (2004). "Altered expression of NDST-1 messenger RNA in puromycin aminonucleoside nephrosis". J. Lab. Clin. Med. 143 (2): 106–14. doi:10.1016/j.lab.2003.10.012. PMID 14966466.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.