NCKAP1

Nck-associated protein 1 is a protein that in humans is encoded by the NCKAP1 gene.[5][6][7][8]

NCKAP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3P8C, 4N78
Identifiers
Aliases	NCKAP1, HEM2, NAP1, NAP125, p125Nap1, NCK associated protein 1
External IDs	OMIM: 604891 MGI: 1355333 HomoloGene: 8384 GeneCards: NCKAP1
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q32.1 Start 182,909,115 bp[1] End 183,038,858 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2 C3|2 48.21 cM Start 80,500,512 bp[2] End 80,581,380 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • Rac GTPase binding • GO:0032403 macromolecular complex binding • GO:0001948 protein binding Cellular component • integral component of membrane • cytosol • cell projection • membrane • SCAR complex • focal adhesion • cell membrane • extracellular exosome • lamellipodium membrane • ruffle • lamellipodium • filamentous actin Biological process • Fc-gamma receptor signaling pathway involved in phagocytosis • positive regulation of Arp2/3 complex-mediated actin nucleation • central nervous system development • vascular endothelial growth factor receptor signaling pathway • Rac protein signal transduction • GO:0022415 viral process • apoptotic process • positive regulation of lamellipodium assembly • positive regulation of actin filament polymerization • cell morphogenesis • cell migration • cell projection assembly • GO:0033109 cortical actin cytoskeleton organization • neuron projection morphogenesis Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10787	50884
Ensembl	ENSG00000061676	ENSMUSG00000027002
UniProt	Q9Y2A7	P28660
RefSeq (mRNA)	NM_013436 NM_205842	NM_001290745 NM_016965 NM_001355410 NM_001355411
RefSeq (protein)	NP_038464 NP_995314	NP_001277674 NP_058661 NP_001342339 NP_001342340
Location (UCSC)	Chr 2: 182.91 – 183.04 Mb	Chr 2: 80.5 – 80.58 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

NCKAP1 has been shown to interact with RAC1[9] and ABI1.[10]

References

1. GRCh38: Ensembl release 89: ENSG00000061676 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027002 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Suzuki T, Nishiyama K, Yamamoto A, Inazawa J, Iwaki T, Yamada T, Kanazawa I, Sakaki Y (Jan 2000). "Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease". Genomics. 63 (2): 246–54. doi:10.1006/geno.1999.6053. PMID 10673335.
6. Eden S, Rohatgi R, Podtelejnikov AV, Mann M, Kirschner MW (Aug 2002). "Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck". Nature. 418 (6899): 790–3. Bibcode:2002Natur.418..790E. doi:10.1038/nature00859. PMID 12181570.
7. Matuoka K, Miki H, Takahashi K, Takenawa T (Oct 1997). "A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs". Biochemical and Biophysical Research Communications. 239 (2): 488–92. doi:10.1006/bbrc.1997.7492. PMID 9344857.
8. "Entrez Gene: NCKAP1 NCK-associated protein 1".
9. Kitamura Y, Kitamura T, Sakaue H, Maeda T, Ueno H, Nishio S, Ohno S, Osada SI, Sakaue M, Ogawa W, Kasuga M (Mar 1997). "Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac". The Biochemical Journal. 322 (Pt 3): 873–8. doi:10.1042/bj3220873. PMC 1218269. PMID 9148763.
10. Yamamoto A, Suzuki T, Sakaki Y (Jun 2001). "Isolation of hNap1BP which interacts with human Nap1 (NCKAP1) whose expression is down-regulated in Alzheimer's disease". Gene. 271 (2): 159–69. doi:10.1016/S0378-1119(01)00521-2. PMID 11418237.

Further reading

• Kitamura T, Kitamura Y, Yonezawa K, Totty NF, Gout I, Hara K, Waterfield MD, Sakaue M, Ogawa W, Kasuga M (Feb 1996). "Molecular cloning of p125Nap1, a protein that associates with an SH3 domain of Nck". Biochemical and Biophysical Research Communications. 219 (2): 509–14. doi:10.1006/bbrc.1996.0264. PMID 8605018.
• Kobayashi K, Kuroda S, Fukata M, Nakamura T, Nagase T, Nomura N, Matsuura Y, Yoshida-Kubomura N, Iwamatsu A, Kaibuchi K (Jan 1998). "p140Sra-1 (specifically Rac1-associated protein) is a novel specific target for Rac1 small GTPase". The Journal of Biological Chemistry. 273 (1): 291–5. doi:10.1074/jbc.273.1.291. PMID 9417078.
• Witke W, Podtelejnikov AV, Di Nardo A, Sutherland JD, Gurniak CB, Dotti C, Mann M (Feb 1998). "In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly". The EMBO Journal. 17 (4): 967–76. doi:10.1093/emboj/17.4.967. PMC 1170446. PMID 9463375.
• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Feb 1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
• Yamamoto A, Suzuki T, Sakaki Y (Jun 2001). "Isolation of hNap1BP which interacts with human Nap1 (NCKAP1) whose expression is down-regulated in Alzheimer's disease". Gene. 271 (2): 159–69. doi:10.1016/S0378-1119(01)00521-2. PMID 11418237.
• Salazar MA, Kwiatkowski AV, Pellegrini L, Cestra G, Butler MH, Rossman KL, Serna DM, Sondek J, Gertler FB, De Camilli P (Dec 2003). "Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeleton". The Journal of Biological Chemistry. 278 (49): 49031–43. doi:10.1074/jbc.M308104200. PMID 14506234.
• Fujita F, Taniguchi Y, Kato T, Narita Y, Furuya A, Ogawa T, Sakurai H, Joh T, Itoh M, Delhase M, Karin M, Nakanishi M (Nov 2003). "Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling". Molecular and Cellular Biology. 23 (21): 7780–93. doi:10.1128/MCB.23.21.7780-7793.2003. PMC 207563. PMID 14560022.
• Innocenti M, Zucconi A, Disanza A, Frittoli E, Areces LB, Steffen A, Stradal TE, Di Fiore PP, Carlier MF, Scita G (Apr 2004). "Abi1 is essential for the formation and activation of a WAVE2 signalling complex". Nature Cell Biology. 6 (4): 319–27. doi:10.1038/ncb1105. PMID 15048123.
• Mayne M, Moffatt T, Kong H, McLaren PJ, Fowke KR, Becker KG, Namaka M, Schenck A, Bardoni B, Bernstein CN, Melanson M (Apr 2004). "CYFIP2 is highly abundant in CD4+ cells from multiple sclerosis patients and is involved in T cell adhesion". European Journal of Immunology. 34 (4): 1217–27. doi:10.1002/eji.200324726. PMID 15048733.
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (Aug 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Current Biology. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Chen W, Tang Z, Fortina P, Patel P, Addya S, Surrey S, Acheampong EA, Mukhtar M, Pomerantz RJ (Mar 2005). "Ethanol potentiates HIV-1 gp120-induced apoptosis in human neurons via both the death receptor and NMDA receptor pathways". Virology. 334 (1): 59–73. doi:10.1016/j.virol.2005.01.014. PMID 15749123.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.