Micro-incineration

Microincineration is a technique to determine the manner and distribution of mineral elements, e.g., calcium (Ca), potassium (K), sodium (Na), magnesium (Mg), iron (Fe), silicion (Si), etc., in biological cells, biological tissues and organs. Slide preparation of tissues can be used. The organic matter is vaporised by heating and nature and position of mineral ash is determined microscopically. Aqueous or cryo-EM fixed tissue materials can also be examined under transmission and scanning electron microscopy (TEM & SEM). The ashing procedure produces cellular oxidised-residues rich in Na₂O, CaO, MgO, Fe₂O₃, SiO₂, Ca(PO₄)₂, Mg(PO₄)₂, etc., which are detected by X-ray microanalysis with 2-4 times sensitivity gained after incineration of sample, due to increased mineral concentration and reduced nonspecific background radiation.

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http://jcb.rupress.org/content/39/1/55.full.pdf

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