LFNG

LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, also known as LFNG and Lunatic Fringe,[5][6] is a human gene.[7]

LFNG
Identifiers
AliasesLFNG, SCDO3, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
External IDsOMIM: 602576 MGI: 1095413 HomoloGene: 22475 GeneCards: LFNG
Gene location (Human)
Chr.Chromosome 7 (human)[1]
Band7p22.3Start2,512,529 bp[1]
End2,529,177 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

3955

16848

Ensembl

ENSG00000106003

ENSMUSG00000029570

UniProt

Q8NES3

O09010

RefSeq (mRNA)

NM_002304
NM_001040167
NM_001040168
NM_001166355

NM_008494

RefSeq (protein)

NP_001035257
NP_001035258
NP_001159827
NP_002295

NP_032520

Location (UCSC)Chr 7: 2.51 – 2.53 MbChr 5: 140.61 – 140.62 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

This gene encodes a member of the glycosyltransferase superfamily. The encoded protein is a single-pass type II Golgi membrane protein that functions as a fucose-specific glycosyltransferase, adding an N-acetylglucosamine to the fucose residue of a group of signaling receptors involved in regulating cell fate decisions during development. Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Alternatively spliced transcript variants that encode different isoforms have been described, however, not all variants have been fully characterized.[7]

Function

Lunatic Fringe (Lfng) is a gene whose role in embryonic development is to establish the anterior boundary of somites, which will eventually develop in vertebrae, ribs, and dermis.[8] Lunatic Fringe responds to certain threshold ratios of retinoic acid and FGF-8 in order to mark the anterior boundary of somites while another transcription factor, Hairy, responds to different threshold ratios of retinoic acid and FGF-8 to form the posterior boundaries of somites.[9]

Clinical significance

A defect associated with Lfng mutations is spondylocostal dysostosis. Spondylocostal dysostosis is characterized by segmentation problems in the developing vertebrae resulting in fusion or lack of vertebrae along with abnormalities in the ribs.[10] Clinically, spondylocostal dysostosis presents as a shortened neck and trunk relative total height and a mild form of scoliosis. Respiratory problems are also common in spondylocostal dysostosis because of the shortened trunk.

A knockout model for Lfng has been created in mice, and without Lfng, mice have shorter tails, and impaired rib, lung, and somite development. A deficiency of Lfng in male mice has also been associated with lack of spermatozoa in the epididymis of many mice; however, spermatogenesis was not impaired. Rather, the male mice were subfertile.[11] In female mice, Lfng deficiency led to infertility because of abnormal folliculogenesis. Further examination showed that oocytes from these female mice did not complete meiotic maturation.[12] However, there are other studies that contradict this stating that not all female mice deficient of Lfng are infertile. A possible explanation for this difference between these studies is that the Lfng alleles were functional different, however, this is unlikely. More likely is that this discrepancy results from differences in the genetic background of the mice or husbandry and colony conditions.[13]

Impact of mutation

Lunatic Fringe is a transcription factor that plays a crucial role in the development of the somites. Somites give rise to the skeletal muscle, the axial skeleton, the tendons, and the dorsal dermis. The somites are formed via the clock-wave front model, and as each somite is formed, each cell receives a burst of FGF8 (a signaling molecule). Somites are formed anterior to posterior, and since FGF8 has a short half-life, this leads to a greater concentration of FGF8 in the posterior, and a lesser concentration in the anterior. Lunatic fringe responds to the lower concentration of FGF8 in the anterior and leads these cells to their developmental fate. Mutation of the Lunatic Fringe gene can cause severe Spondylocostal Dysostosis, which involves vertebral segmentation defects and rib abnormalities. A mutation was discovered in which a conservative phenylalanine close to the active site of the enzyme mutates, leading to the enzymatic inactivation of Lunatic Fringe. A “knock-out” model has been created using mice. In mice, Lunatic Fringe plays a crucial role in the Notch signaling pathway during the formation of somites, and a mutation in this gene leads to somites with irregular shapes and a defect in the anterior-posterior formation.

[10]

[14]

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References
  1. GRCh38: Ensembl release 89: ENSG00000106003 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000029570 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Egan S, Herbrick JA, Tsui LC, Cohen B, Flock G, Beatty B, Scherer SW (December 1998). "Mapping of the human Lunatic Fringe (LFNG) gene to 7p22 and Manic Fringe (MFNG) to 22q12". Genomics. 54 (3): 576–7. doi:10.1006/geno.1998.5559. PMID 9878264.
  6. "Troublesome gene names get the boot", 6 November 2006, Michael Hopkin, nature.com
  7. "Entrez Gene: LFNG LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase".
  8. Serth K, Schuster-Gossler K, Cordes R, Gossler A (April 2003). "Transcriptional oscillation of lunatic fringe is essential for somitogenesis". Genes & Development. 17 (7): 912–25. doi:10.1101/gad.250603. PMC 196028. PMID 12670869.
  9. Shifley ET, Vanhorn KM, Perez-Balaguer A, Franklin JD, Weinstein M, Cole SE (March 2008). "Oscillatory lunatic fringe activity is crucial for segmentation of the anterior but not posterior skeleton". Development. 135 (5): 899–908. doi:10.1242/dev.006742. PMID 18234727.
  10. Sparrow DB, Chapman G, Wouters MA, Whittock NV, Ellard S, Fatkin D, Turnpenny PD, Kusumi K, Sillence D, Dunwoodie SL (January 2006). "Mutation of the LUNATIC FRINGE gene in humans causes spondylocostal dysostosis with a severe vertebral phenotype". American Journal of Human Genetics. 78 (1): 28–37. doi:10.1086/498879. PMC 1380221. PMID 16385447.
  11. Hahn KL, Beres B, Rowton MJ, Skinner MK, Chang Y, Rawls A, Wilson-Rawls J (January 2009). "A deficiency of lunatic fringe is associated with cystic dilation of the rete testis". Reproduction. 137 (1): 79–93. doi:10.1530/REP-08-0207. PMC 5739036. PMID 18801836.
  12. Hahn KL, Johnson J, Beres BJ, Howard S, Wilson-Rawls J (February 2005). "Lunatic fringe null female mice are infertile due to defects in meiotic maturation". Development. 132 (4): 817–28. doi:10.1242/dev.01601. PMID 15659488.
  13. Xu J, Norton CR, Gridley T (February 2006). "Not all lunatic fringe null female mice are infertile". Development. 133 (4): 579, author reply 579–80. doi:10.1242/dev.02221. PMID 16436621.
  14. Zhang N, Gridley T (July 1998). "Defects in somite formation in lunatic fringe-deficient mice". Nature. 394 (6691): 374–7. doi:10.1038/28625. PMID 9690472.

Further reading

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