Calcium-activated potassium channel subunit alpha-1

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (K_Ca1.1), or BK channel alpha subunit,[5] is a voltage gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes.[6]

KCNMA1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2K44, 3MT5, 3NAF
Identifiers
Aliases	KCNMA1, BKTM, KCa1.1, MaxiK, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, mSLO1, hSlo, potassium calcium-activated channel subfamily M alpha 1, CADEDS, PNKD3, IEG16, LIWAS
External IDs	OMIM: 600150 MGI: 99923 HomoloGene: 1693 GeneCards: KCNMA1
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10q22.3 Start 76,869,601 bp[1] End 77,638,369 bp[1]
Gene location (Mouse) Chr. Chromosome 14 (mouse)[2] Band 14|14 A3 Start 23,289,431 bp[2] End 24,014,491 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • potassium channel activity • metal ion binding • voltage-gated ion channel activity • ion channel activity • GO:0001948 protein binding • voltage-gated potassium channel activity • actin binding • calcium-activated potassium channel activity • large conductance calcium-activated potassium channel activity • identical protein binding • outward rectifier potassium channel activity Cellular component • integral component of membrane • membrane • voltage-gated potassium channel complex • cell membrane • apical plasma membrane • caveola • postsynaptic membrane Biological process • response to hypoxia • urination • regulation of membrane potential • regulation of ion transmembrane transport • negative regulation of cell volume • ion transport • cellular potassium ion homeostasis • response to carbon monoxide • response to osmotic stress • response to calcium ion • potassium ion transport • transmembrane transport • positive regulation of apoptotic process • smooth muscle contraction involved in micturition • potassium ion transmembrane transport • relaxation of vascular smooth muscle • ion transmembrane transport Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	3778	16531
Ensembl	ENSG00000156113	ENSMUSG00000063142
UniProt	Q12791	Q08460
RefSeq (mRNA)	NM_001014797 NM_001161352 NM_001161353 NM_001271518 NM_001271519 NM_001271520 NM_001271521 NM_001271522 NM_002247 NM_001322829 NM_001322830 NM_001322832 NM_001322835 NM_001322836 NM_001322837 NM_001322838 NM_001322839	NM_001253358 NM_001253359 NM_001253360 NM_001253361 NM_001253362 NM_001253363 NM_001253364 NM_001253365 NM_001253366 NM_001253367 NM_001253368 NM_001253369 NM_001253370 NM_001253371 NM_001253372 NM_001253373 NM_001253374 NM_001253375 NM_001253376 NM_001253377 NM_001253378 NM_010610
RefSeq (protein)	NP_001014797 NP_001154824 NP_001154825 NP_001258447 NP_001258448 NP_001258449 NP_001258450 NP_001258451 NP_001309758 NP_001309759 NP_001309761 NP_001309764 NP_001309765 NP_001309766 NP_001309767 NP_001309768 NP_002238 NP_001258448.1	NP_001240287 NP_001240288 NP_001240289 NP_001240290 NP_001240291 NP_001240292 NP_001240293 NP_001240294 NP_001240295 NP_001240296 NP_001240297 NP_001240298 NP_001240299 NP_001240300 NP_001240301 NP_001240302 NP_001240303 NP_001240304 NP_001240305 NP_001240306 NP_001240307 NP_034740
Location (UCSC)	Chr 10: 76.87 – 77.64 Mb	Chr 14: 23.29 – 24.01 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

BK channels are activated (opened) by changes in membrane electrical potential and/or by increases in concentration of intracellular calcium ion (Ca²⁺).[7][8] Opening of BK channels allows K⁺ to passively flow through the channel, down the electrochemical gradient. Under typical physiological conditions, this results in an efflux of K⁺ from the cell, which leads to cell membrane hyperpolarization (a decrease in the electrical potential across the cell membrane) and a decrease in cell excitability (a decrease in the probability that the cell will transmit an action potential).

BK channels are essential for the regulation of several key physiological processes including smooth muscle tone and neuronal excitability.[6] They control the contraction of smooth muscle and are involved with the electrical tuning of hair cells in the cochlea. BK channels also contribute to the behavioral effects of ethanol in the worm C. elegans under high concentrations (> 100 mM, or approximately 0.50% BAC).[9] It remains to be determined if BK channels contribute to intoxication in humans.

Structure

BK channels have a tetrameric structure. Each monomer of the channel-forming alpha subunit is the product of the KCNMA1 gene. Modulatory beta subunits (encoded by KCNMB1, KCNMB2, KCNMB3, or KCNMB4) can associate with the tetrameric channel. Alternatively spliced transcript variants encoding different isoforms have been identified.[6]

Each BK channel alpha subunit consists of (from N- to C-terminal):

1. A unique transmembrane domain (S0)[10] that precedes the 6 transmembrane domains (S1-S6) conserved in all voltage-dependent K⁺ channels.
2. A voltage sensing domain (S1-S4).
3. A K⁺ channel pore domain (S5, selectivity filter, and S6).
4. A cytoplasmic C-terminal domain (CTD) consisting of a pair of RCK domains that assemble into an octameric gating ring on the intracellular side of the tetrameric channel.[8][11][12][13][14][15][16] The CTD contains four primary binding sites for Ca²⁺, called "calcium bowls", encoded within the second RCK domain of each monomer.[8][11][15][16]

Calcium-activated BK potassium channel alpha subunit
Identifiers
Symbol	BK_channel_a
Pfam	PF03493
InterPro	IPR003929
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Available X-ray structures include:

• 3U6N​ – Open structure of the BK channel gating ring[16]
• 3MT5​ – Crystal structure of the human BK gating apparatus[8]
• 3NAF​ – Structure of the intracellular gating ring from the human high-conductance Ca²⁺ gated K⁺ channel (BK Channel)[11]

Pharmacology

BK channels are pharmacological targets for the treatment of stroke. Various pharmaceutical companies developed synthetic molecules activating these channels[17] in order to prevent excessive neurotoxic calcium entry in neurons.[18] But BMS-204352 (MaxiPost) a molecule developed by Bristol-Myers Squibb failed to improve clinical outcome in stroke patients compared to placebo.[19] BK channels have also been found to be activated by exogenous pollutants and endogenous gazotransmitters carbon monoxide[20][21] and hydrogen sulphide.[22]

BK channels are blocked by tetraethylammonium (TEA), paxilline[23] and iberiotoxin.[24]

Related conditions

Researchers have identified a rare disease in humans caused by mutations in the gene.  KCNMA1-linked channelopathy can cause neurological conditions like seizures and movement disorders.[25] An episode of the TV show based on the Diagnosis column in the New York Times was about a young girl with a KCNMA1 disorder that caused transient episodes of muscle weakness.[26]

See also

• BK channel
• Calcium-activated potassium channel
• Voltage-gated potassium channel

References

1. GRCh38: Ensembl release 89: ENSG00000156113 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000063142 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "HomoloGene - NCBI". www.ncbi.nlm.nih.gov.
6. "Entrez Gene: KCNMA1 potassium large conductance calcium-activated channel, subfamily M, alpha member 1".
7. Miller C (2000). "An overview of the potassium channel family". Genome Biology. 1 (4): REVIEWS0004. doi:10.1186/gb-2000-1-4-reviews0004. PMC 138870. PMID 11178249.
8. Yuan P, Leonetti MD, Pico AR, Hsiung Y, MacKinnon R (July 2010). "Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution". Science. 329 (5988): 182–6. Bibcode:2010Sci...329..182Y. doi:10.1126/science.1190414. PMC 3022345. PMID 20508092.
9. Davies AG, Pierce-Shimomura JT, Kim H, VanHoven MK, Thiele TR, Bonci A, et al. (December 2003). "A central role of the BK potassium channel in behavioral responses to ethanol in C. elegans". Cell. 115 (6): 655–66. doi:10.1016/S0092-8674(03)00979-6. PMID 14675531.
10. Wallner M, Meera P, Toro L (December 1996). "Determinant for beta-subunit regulation in high-conductance voltage-activated and Ca(2+)-sensitive K+ channels: an additional transmembrane region at the N terminus". Proceedings of the National Academy of Sciences of the United States of America. 93 (25): 14922–7. Bibcode:1996PNAS...9314922W. doi:10.1073/pnas.93.25.14922. PMC 26238. PMID 8962157.
11. Wu Y, Yang Y, Ye S, Jiang Y (July 2010). "Structure of the gating ring from the human large-conductance Ca(2+)-gated K(+) channel". Nature. 466 (7304): 393–7. Bibcode:2010Natur.466..393W. doi:10.1038/nature09252. PMC 2910425. PMID 20574420.
12. Jiang Y, Pico A, Cadene M, Chait BT, MacKinnon R (March 2001). "Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel". Neuron. 29 (3): 593–601. doi:10.1016/S0896-6273(01)00236-7. PMID 11301020.
13. Pico A. 2003. RCK domain model of calcium activation in BK channels. PhD thesis. The Rockfeller University, New York.
14. Yusifov T, Savalli N, Gandhi CS, Ottolia M, Olcese R (January 2008). "The RCK2 domain of the human BKCa channel is a calcium sensor". Proceedings of the National Academy of Sciences of the United States of America. 105 (1): 376–81. Bibcode:2008PNAS..105..376Y. doi:10.1073/pnas.0705261105. PMC 2224220. PMID 18162557.
15. Schreiber M, Salkoff L (September 1997). "A novel calcium-sensing domain in the BK channel". Biophysical Journal. 73 (3): 1355–63. Bibcode:1997BpJ....73.1355S. doi:10.1016/S0006-3495(97)78168-2. PMC 1181035. PMID 9284303.
16. Yuan P, Leonetti MD, Hsiung Y, MacKinnon R (December 2011). "Open structure of the Ca2+ gating ring in the high-conductance Ca2+-activated K+ channel". Nature. 481 (7379): 94–7. Bibcode:2012Natur.481...94Y. doi:10.1038/nature10670. PMC 3319005. PMID 22139424.
17. Gribkoff VK, Winquist RJ (May 2005). "Voltage-gated cation channel modulators for the treatment of stroke". Expert Opinion on Investigational Drugs. 14 (5): 579–92. doi:10.1517/13543784.14.5.579. PMID 15926865.
18. Gribkoff VK, Starrett JE, Dworetzky SI (April 2001). "Maxi-K potassium channels: form, function, and modulation of a class of endogenous regulators of intracellular calcium". The Neuroscientist. 7 (2): 166–77. doi:10.1177/107385840100700211. PMID 11496927.
19. Jensen BS (2002). "BMS-204352: a potassium channel opener developed for the treatment of stroke". CNS Drug Reviews. 8 (4): 353–60. doi:10.1111/j.1527-3458.2002.tb00233.x. PMC 6741660. PMID 12481191.
20. Dubuis E, Potier M, Wang R, Vandier C (February 2005). "Continuous inhalation of carbon monoxide attenuates hypoxic pulmonary hypertension development presumably through activation of BKCa channels". Cardiovascular Research. 65 (3): 751–61. doi:10.1016/j.cardiores.2004.11.007. PMID 15664403.
21. Hou S, Xu R, Heinemann SH, Hoshi T (March 2008). "The RCK1 high-affinity Ca2+ sensor confers carbon monoxide sensitivity to Slo1 BK channels". Proceedings of the National Academy of Sciences of the United States of America. 105 (10): 4039–43. Bibcode:2008PNAS..105.4039H. doi:10.1073/pnas.0800304105. PMC 2268785. PMID 18316727.
22. Sitdikova GF, Weiger TM, Hermann A (February 2010). "Hydrogen sulfide increases calcium-activated potassium (BK) channel activity of rat pituitary tumor cells". Pflügers Archiv. 459 (3): 389–97. doi:10.1007/s00424-009-0737-0. PMID 19802723.
23. "Paxilline, from Fermentek".
24. Candia S, Garcia ML, Latorre R (August 1992). "Mode of action of iberiotoxin, a potent blocker of the large conductance Ca(2+)-activated K+ channel". Biophysical Journal. 63 (2): 583–90. Bibcode:1992BpJ....63..583C. doi:10.1016/S0006-3495(92)81630-2. PMC 1262182. PMID 1384740.
25. Bailey CS, Moldenhauer HJ, Park SM, Keros S, Meredith AL (October 2019). "KCNMA1-linked channelopathy". The Journal of General Physiology. 151 (10): 1173–1189. doi:10.1085/jgp.201912457. PMC 6785733. PMID 31427379.
26. Sanders L (2018-09-11). "A Diagnosis Update: New Information on a Young Girl's Rare Genetic Condition". The New York Times. Retrieved 2019-11-02.

Further reading

• Magleby KL (February 2003). "Gating mechanism of BK (Slo1) channels: so near, yet so far". The Journal of General Physiology. 121 (2): 81–96. doi:10.1085/jgp.20028721. PMC 2217328. PMID 12566537.
• Wei AD, Gutman GA, Aldrich R, Chandy KG, Grissmer S, Wulff H (December 2005). "International Union of Pharmacology. LII. Nomenclature and molecular relationships of calcium-activated potassium channels". Pharmacological Reviews. 57 (4): 463–72. doi:10.1124/pr.57.4.9. PMID 16382103.
• McCobb DP, Fowler NL, Featherstone T, Lingle CJ, Saito M, Krause JE, Salkoff L (September 1995). "A human calcium-activated potassium channel gene expressed in vascular smooth muscle". The American Journal of Physiology. 269 (3 Pt 2): H767-77. doi:10.1152/ajpheart.1995.269.3.H767. PMID 7573516.
• Butler A, Tsunoda S, McCobb DP, Wei A, Salkoff L (July 1993). "mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels". Science. 261 (5118): 221–4. Bibcode:1993Sci...261..221B. doi:10.1126/science.7687074. PMID 7687074.
• Dworetzky SI, Trojnacki JT, Gribkoff VK (November 1994). "Cloning and expression of a human large-conductance calcium-activated potassium channel". Brain Research. Molecular Brain Research. 27 (1): 189–93. doi:10.1016/0169-328X(94)90203-8. PMID 7877450.
• Pallanck L, Ganetzky B (August 1994). "Cloning and characterization of human and mouse homologs of the Drosophila calcium-activated potassium channel gene, slowpoke". Human Molecular Genetics. 3 (8): 1239–43. doi:10.1093/hmg/3.8.1239. PMID 7987297.
• Tseng-Crank J, Foster CD, Krause JD, Mertz R, Godinot N, DiChiara TJ, Reinhart PH (December 1994). "Cloning, expression, and distribution of functionally distinct Ca(2+)-activated K+ channel isoforms from human brain". Neuron. 13 (6): 1315–30. doi:10.1016/0896-6273(94)90418-9. PMID 7993625.
• Knaus HG, Folander K, Garcia-Calvo M, Garcia ML, Kaczorowski GJ, Smith M, Swanson R (June 1994). "Primary sequence and immunological characterization of beta-subunit of high conductance Ca(2+)-activated K+ channel from smooth muscle". The Journal of Biological Chemistry. 269 (25): 17274–8. PMID 8006036.
• Meera P, Wallner M, Jiang Z, Toro L (March 1996). "A calcium switch for the functional coupling between alpha (hslo) and beta subunits (KV,Ca beta) of maxi K channels". FEBS Letters. 382 (1–2): 84–8. doi:10.1016/0014-5793(96)00151-2. PMID 8612769.
• Wallner M, Meera P, Ottolia M, Kaczorowski GJ, Latorre R, Garcia ML, et al. (1996). "Characterization of and modulation by a beta-subunit of a human maxi KCa channel cloned from myometrium". Receptors & Channels. 3 (3): 185–99. PMID 8821792.
• Meera P, Wallner M, Song M, Toro L (December 1997). "Large conductance voltage- and calcium-dependent K+ channel, a distinct member of voltage-dependent ion channels with seven N-terminal transmembrane segments (S0-S6), an extracellular N terminus, and an intracellular (S9-S10) C terminus". Proceedings of the National Academy of Sciences of the United States of America. 94 (25): 14066–71. Bibcode:1997PNAS...9414066M. doi:10.1073/pnas.94.25.14066. PMC 28433. PMID 9391153.
• Díaz L, Meera P, Amigo J, Stefani E, Alvarez O, Toro L, Latorre R (December 1998). "Role of the S4 segment in a voltage-dependent calcium-sensitive potassium (hSlo) channel". The Journal of Biological Chemistry. 273 (49): 32430–6. doi:10.1074/jbc.273.49.32430. PMID 9829973.
• Wallner M, Meera P, Toro L (March 1999). "Molecular basis of fast inactivation in voltage and Ca2+-activated K+ channels: a transmembrane beta-subunit homolog". Proceedings of the National Academy of Sciences of the United States of America. 96 (7): 4137–42. Bibcode:1999PNAS...96.4137W. doi:10.1073/pnas.96.7.4137. PMC 22433. PMID 10097176.
• Valverde MA, Rojas P, Amigo J, Cosmelli D, Orio P, Bahamonde MI, et al. (September 1999). "Acute activation of Maxi-K channels (hSlo) by estradiol binding to the beta subunit". Science. 285 (5435): 1929–31. doi:10.1126/science.285.5435.1929. PMID 10489376.
• Brenner R, Jegla TJ, Wickenden A, Liu Y, Aldrich RW (March 2000). "Cloning and functional characterization of novel large conductance calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4". The Journal of Biological Chemistry. 275 (9): 6453–61. doi:10.1074/jbc.275.9.6453. PMID 10692449.
• Liu QH, Williams DA, McManus C, Baribaud F, Doms RW, Schols D, et al. (April 2000). "HIV-1 gp120 and chemokines activate ion channels in primary macrophages through CCR5 and CXCR4 stimulation". Proceedings of the National Academy of Sciences of the United States of America. 97 (9): 4832–7. Bibcode:2000PNAS...97.4832L. doi:10.1073/pnas.090521697. PMC 18318. PMID 10758170.
• Quirk JC, Reinhart PH (October 2001). "Identification of a novel tetramerization domain in large conductance K(ca) channels". Neuron. 32 (1): 13–23. doi:10.1016/S0896-6273(01)00444-5. PMID 11604135.
• Soto MA, González C, Lissi E, Vergara C, Latorre R (March 2002). "Ca(2+)-activated K+ channel inhibition by reactive oxygen species". American Journal of Physiology. Cell Physiology. 282 (3): C461-71. doi:10.1152/ajpcell.00167.2001. PMID 11832330.
• Wang YW, Ding JP, Xia XM, Lingle CJ (March 2002). "Consequences of the stoichiometry of Slo1 alpha and auxiliary beta subunits on functional properties of large-conductance Ca2+-activated K+ channels". The Journal of Neuroscience. 22 (5): 1550–61. doi:10.1523/JNEUROSCI.22-05-01550.2002. PMC 6758889. PMID 11880485.

External links

Classification	D OMIM: 600150

• Meredith Lab