InaD-like protein

InaD-like protein is a protein that in humans is encoded by the PATJ gene.[5][6][7]

PATJ
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1VF6, 2D92, 2DAZ, 2DB5, 2DLU, 2DM8, 2DMZ, 2EHR, 4Q2N
Identifiers
Aliases	PATJ, Cipp, InaD-like, hINADL, INADL, crumbs cell polarity complex component, PATJ crumbs cell polarity complex component
External IDs	OMIM: 603199 MGI: 1277960 HomoloGene: 72199 GeneCards: PATJ
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1p31.3 Start 61,742,406 bp[1] End 62,178,675 bp[1]
Gene location (Mouse) Chr. Chromosome 4 (mouse)[2] Band 4|4 C6 Start 98,395,785 bp[2] End 98,719,603 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding Cellular component • cytoplasm • perinuclear region of cytoplasm • cell membrane • apical part of cell • extracellular exosome • apical plasma membrane • membrane • bicellular tight junction • centrosome • microtubule organizing center • cytosol • cell junction • macromolecular complex Biological process • GO:0007243 intracellular signal transduction • bicellular tight junction assembly Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10207	12695
Ensembl	ENSG00000132849	ENSMUSG00000061859
UniProt	Q8NI35	Q63ZW7
RefSeq (mRNA)	NM_005799 NM_176877 NM_176878 NM_001350145	NM_001005784 NM_001005787 NM_007704 NM_172696 NM_001355177
RefSeq (protein)	NP_795352 NP_001337074	NP_001005784 NP_001005787 NP_031730 NP_766284 NP_001342106
Location (UCSC)	Chr 1: 61.74 – 62.18 Mb	Chr 4: 98.4 – 98.72 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.[7]

Interactions

INADL has been shown to interact with MPP5.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000132849 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000061859 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Philipp S, Flockerzi V (Sep 1997). "Molecular characterization of a novel human PDZ domain protein with homology to INAD from Drosophila melanogaster". FEBS Lett. 413 (2): 243–8. doi:10.1016/S0014-5793(97)00877-6. PMID 9280290.
6. Soejima H, Kawamoto S, Akai J, Miyoshi O, Arai Y, Morohka T, Matsuo S, Niikawa N, Kimura A, Okubo K, Mukai T (May 2001). "Isolation of novel heart-specific genes using the BodyMap database". Genomics. 74 (1): 115–20. doi:10.1006/geno.2001.6527. PMID 11374908.
7. "Entrez Gene: INADL InaD-like (Drosophila)".
8. Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B (Apr 2002). "The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost". J. Cell Biol. 157 (1): 161–72. doi:10.1083/jcb.200109010. PMC 2173254. PMID 11927608.

Further reading

• Fanning AS, Anderson JM (1999). "PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane". J. Clin. Invest. 103 (6): 767–72. doi:10.1172/JCI6509. PMC 408156. PMID 10079096.
• Lennon G, Auffray C, Polymeropoulos M, Soares MB (1996). "The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression". Genomics. 33 (1): 151–2. doi:10.1006/geno.1996.0177. PMID 8617505.
• Kurschner C, Mermelstein PG, Holden WT, Surmeier DJ (1998). "CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins". Mol. Cell. Neurosci. 11 (3): 161–72. doi:10.1006/mcne.1998.0679. PMID 9647694.
• Vaccaro P, Brannetti B, Montecchi-Palazzi L, Philipp S, Helmer Citterich M, Cesareni G, Dente L (2001). "Distinct binding specificity of the multiple PDZ domains of INADL, a human protein with homology to INAD from Drosophila melanogaster". J. Biol. Chem. 276 (45): 42122–30. doi:10.1074/jbc.M104208200. PMID 11509564.
• Anzai N, Deval E, Schaefer L, Friend V, Lazdunski M, Lingueglia E (2002). "The multivalent PDZ domain-containing protein CIPP is a partner of acid-sensing ion channel 3 in sensory neurons". J. Biol. Chem. 277 (19): 16655–61. doi:10.1074/jbc.M201087200. PMID 11872753.
• Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B (2002). "The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost". J. Cell Biol. 157 (1): 161–72. doi:10.1083/jcb.200109010. PMC 2173254. PMID 11927608.
• Lemmers C, Médina E, Delgrossi MH, Michel D, Arsanto JP, Le Bivic A (2002). "hINADl/PATJ, a homolog of discs lost, interacts with crumbs and localizes to tight junctions in human epithelial cells". J. Biol. Chem. 277 (28): 25408–15. doi:10.1074/jbc.M202196200. PMID 11964389.
• Roh MH, Liu CJ, Laurinec S, Margolis B (2002). "The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions". J. Biol. Chem. 277 (30): 27501–9. doi:10.1074/jbc.M201177200. PMID 12021270.
• Makarova O, Roh MH, Liu CJ, Laurinec S, Margolis B (2003). "Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1)". Gene. 302 (1–2): 21–9. doi:10.1016/S0378111902010843. PMID 12527193.
• Roh MH, Fan S, Liu CJ, Margolis B (2004). "The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells". J. Cell Sci. 116 (Pt 14): 2895–906. doi:10.1242/jcs.00500. PMID 12771187.
• Li Y, Karnak D, Demeler B, Margolis B, Lavie A (2005). "Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes". EMBO J. 23 (14): 2723–33. doi:10.1038/sj.emboj.7600294. PMC 514954. PMID 15241471.
• Michel D, Arsanto JP, Massey-Harroche D, Béclin C, Wijnholds J, Le Bivic A (2005). "PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells". J. Cell Sci. 118 (Pt 17): 4049–57. doi:10.1242/jcs.02528. PMID 16129888.
• Stiffler MA, Grantcharova VP, Sevecka M, MacBeath G (2007). "Uncovering Quantitative Protein Interaction Networks for Mouse PDZ Domains using Protein Microarrays". J. Am. Chem. Soc. 128 (17): 5913–22. doi:10.1021/ja060943h. PMC 2533859. PMID 16637659.
• Wells CD, Fawcett JP, Traweger A, Yamanaka Y, Goudreault M, Elder K, Kulkarni S, Gish G, Virag C, Lim C, Colwill K, Starostine A, Metalnikov P, Pawson T (2006). "A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells". Cell. 125 (3): 535–48. doi:10.1016/j.cell.2006.02.045. PMID 16678097.

External links

• INADL human gene location in the UCSC Genome Browser.
• INADL human gene details in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: Q8NI35 (InaD-like protein) at the PDBe-KB.