Image Studio Lite
Image Studio™ Lite is free image processing software used for quantitation of Western blot images and images from related experiments.
| Developer(s) | LI-COR Biosciences |
|---|---|
| Stable release | 5.0
|
| Operating system | Windows 7, 8, and 8.1 Mac OS 10.9+ |
| Website | www |
Overview
The primary function of Image Studio Lite is to quantitate relative abundance of proteins on a Western blot or DNA/RNA in an electrophoresis gel from an image of the blot or gel.
History
Release history of Image Studio Lite software:
| Version | Release Date | Compatibility |
|---|---|---|
| Image Studio Lite 5.0 | March 3, 2015 | Windows: 7, 8, 8.1; Mac OS: 10.9 or 10.10 |
| Image Studio Lite 4.0 | March 3, 2014 | Windows: 7, 8, 8.1; Mac OS: 10.8 or 10.9 |
| Image Studio Lite 3.1 | February 1, 2013 | Windows: 7, XP, Vista; Mac OS: 10.6, 10.7, 10.8 |
gollark: I do not understand that sentence ("The alternative is work a political method for political reason.") and it is not pizza, I have had no commercial relations with pizza companies, I am not paid to subliminally advertise pizza, etc.
gollark: I guess maybe in politics/economics/sociology the alternative is something like "lean on human intuition" or "make the correct behaviour magically resolve from self-interest". Not sure how well those actually work.
gollark: - the replication crisis does exist, but it's not like *every paper* has a 50% chance of being wrong - it's mostly in some fields and you can generally estimate which things won't replicate fairly well without much specialized knowledge- science™ agrees on lots of things, just not some highly politicized things- you *can* do RCTs and correlation studies and such, which they seem to be ignoring- some objectivity is better than none- sure, much of pop science is not great, but that doesn't invalidate... all science- they complain about running things based on "trial and error and guesswork", but then don't offer any alternative
gollark: The alternative to basing things on science, I mean. The obvious alternative seems to basically just be guessing?
gollark: What's the alternative? Science is at least *slightly* empirical and right. Also, the video is wrong.
See also
References
- "Image Studio Lite: Tutorial Guide" (PDF). Retrieved 7 March 2014.
- Miller, Luke. "Analyzing western blots with Image Studio Lite". lukemiller.org. Retrieved 7 March 2014.
- "Western Blot Quantification with LI-COR Image Studio Lite". How to WESTERN-BLOT. Retrieved 7 March 2014.
- "Densitometry Tutorial for Image Studio Lite from LI-COR". How to WESTERN-BLOT. Retrieved 7 March 2014.
Further reading
- Atkinson, A. J.; Logantha, S. J. R. J.; Hao, G.; Yanni, J.; Fedorenko, O.; Sinha, A.; Gilbert, S. H.; Benson, A. P.; Buckley, D. L.; Anderson, R. H.; Boyett, M. R.; Dobrzynski, H. (2013). "Functional, Anatomical, and Molecular Investigation of the Cardiac Conduction System and Arrhythmogenic Atrioventricular Ring Tissue in the Rat Heart". Journal of the American Heart Association. 2 (6): e000246. doi:10.1161/JAHA.113.000246. PMC 3886739. PMID 24356527.
- Lo, A. W. H.; Van De Water, K.; Gane, P. J.; Chan, A. W. E.; Steadman, D.; Stevens, K.; Selwood, D. L.; Waksman, G.; Remaut, H. (2013). "Suppression of type 1 pilus assembly in uropathogenic Escherichia coli by chemical inhibition of subunit polymerization". Journal of Antimicrobial Chemotherapy. 69 (4): 1017–1026. doi:10.1093/jac/dkt467. PMC 3956373. PMID 24324225.
- Betz, A; Jayatilaka, S; Joshi, J; Ramanan, S; Debartolo, D; Pylypiw, H; Franke, E (2013). "Chronic exposure to benzyl butyl phthalate (BBP) alters social interaction and fear conditioning in male adult rats: Alterations in amygdalar MeCP2, ERK1/2 and ERα". Neuro Endocrinology Letters. 34 (5): 347–58. PMID 23922038.
- Chen, Y.; Gao, H.; Yin, Q.; Chen, L.; Dong, P.; Zhang, X.; Kang, J. (2013). "ER Stress Activating ATF4/CHOP-TNF-a Signaling Pathway Contributes to Alcohol-Induced Disruption of Osteogenic Lineage of Multipotential Mesenchymal Stem Cell". Cellular Physiology and Biochemistry. 32 (3): 743–54. doi:10.1159/000354476. PMID 24080827.
- Oh, H. S.; Bryant, K. F.; Nieland, T. J. F.; Mazumder, A.; Bagul, M.; Bathe, M.; Root, D. E.; Knipe, D. M. (2014). "A Targeted RNA Interference Screen Reveals Novel Epigenetic Factors That Regulate Herpesviral Gene Expression". mBio. 5: e01086–e01013. doi:10.1128/mBio.01086-13. PMC 3950524. PMID 24496796.
- Lemessurier, K.S.; Häcker, H.; Chi, L.; Tuomanen, E.; Redecke, V. (2013). "Type I Interferon Protects against Pneumococcal Invasive Disease by Inhibiting Bacterial Transmigration across the Lung". PLoS Pathogens. 9 (11): e1003727. doi:10.1371/journal.ppat.1003727. PMC 3820719. PMID 24244159.
- Chen, Y.; Chen, L.; Yin, Q.; Gao, H.; Dong, P.; Zhang, X.; Kang, J. (2013). "Reciprocal Interferences of TNF-α and Wnt1/β-Catenin Signaling Axes Shift Bone Marrow-Derived Stem Cells Towards Osteoblast Lineage after Ethanol Exposure". Cellular Physiology and Biochemistry. 32 (3): 755–765. doi:10.1159/000354477. PMID 24080828.
External links
- Miller, Luke. "Quantifying western blots without expensive commercial quantification software". lukemiller.org. Retrieved 7 March 2014.
- Miller, Luke. "Analyzing gels and western blots with ImageJ". lukemiller.org. Retrieved 7 March 2014.
- "Quantification of Western Blots Using ImageJ". How to WESTERN-BLOT. Retrieved 7 March 2014.
- "Image Studio Lite Software". licor.com. Retrieved 7 March 2014.
- "ImageJ". Retrieved 10 March 2014.
- "MCID Core Digital Imaging Software". Retrieved 10 March 2014.
- "Image Lab™ Software". Retrieved 1 May 2014.
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