Intersectin 2

Intersectin-2 is a protein that in humans is encoded by the ITSN2 gene.[4][5][6]

ITSN2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1J3T, 1UDL, 1UE9, 1UFF, 1UHF, 3GF9, 3JZY, 4IIO
Identifiers
Aliases	ITSN2, PRO2015, SH3D1B, SH3P18, SWA, SWAP, intersectin 2
External IDs	OMIM: 604464 MGI: 1338049 HomoloGene: 22627 GeneCards: ITSN2
Gene location (Mouse) Chr. Chromosome 12 (mouse)[1] Band 12|12 A1.1 Start 4,592,638 bp[1] End 4,713,962 bp[1]
RNA expression pattern More reference expression data
Gene ontology Molecular function • Rho guanyl-nucleotide exchange factor activity • calcium ion binding • GO:0001948 protein binding • metal ion binding Cellular component • cytoplasm • centrosome • extracellular exosome Biological process • positive regulation of dendrite extension • cell differentiation • regulation of Rho protein signal transduction • endocytosis • positive regulation of signal transduction • regulation of molecular function • GO:0022415 viral process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	50618	20403
Ensembl	n/a	ENSMUSG00000020640
UniProt	Q9NZM3	Q9Z0R6
RefSeq (mRNA)	NM_147152 NM_006277 NM_019595	NM_001198968 NM_001198969 NM_011365
RefSeq (protein)	NP_006268 NP_062541 NP_671494 NP_001335110 NP_001335111 NP_001335112 NP_001335113 NP_001335114 NP_001335115	n/a
Location (UCSC)	n/a	Chr 12: 4.59 – 4.71 Mb
PubMed search	[2]	[3]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. Alternatively spliced transcript variants have been found for this gene that encode three distinct isoforms. Additional variants have been found but their full length nature has not been determined.[6]

Interactions

ITSN2 has been shown to interact with Wiskott-Aldrich syndrome protein.[5]

References

1. GRCm38: Ensembl release 89: ENSMUSG00000020640 - Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Pucharcos C, Estivill X, de la Luna S (Aug 2000). "Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis". FEBS Lett. 478 (1–2): 43–51. doi:10.1016/S0014-5793(00)01793-2. PMID 10922467.
5. McGavin MK, Badour K, Hardy LA, Kubiseski TJ, Zhang J, Siminovitch KA (Dec 2001). "The intersectin 2 adaptor links Wiskott Aldrich Syndrome protein (WASp)-mediated actin polymerization to T cell antigen receptor endocytosis". J Exp Med. 194 (12): 1777–87. doi:10.1084/jem.194.12.1777. PMC 2193569. PMID 11748279.
6. "Entrez Gene: ITSN2 intersectin 2".

Further reading

• Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Sparks AB, Hoffman NG, McConnell SJ, et al. (1998). "Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins". Nat. Biotechnol. 14 (6): 741–4. doi:10.1038/nbt0696-741. PMID 9630982.
• Nagase T, Ishikawa K, Kikuno R, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (5): 337–45. doi:10.1093/dnares/6.5.337. PMID 10574462.
• Pucharcos C, Casas C, Nadal M, et al. (2001). "The human intersectin genes and their spliced variants are differentially expressed". Biochim. Biophys. Acta. 1521 (1–3): 1–11. doi:10.1016/s0167-4781(01)00276-7. PMID 11690630.
• Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Chini CC, Chen J (2003). "Human claspin is required for replication checkpoint control". J. Biol. Chem. 278 (32): 30057–62. doi:10.1074/jbc.M301136200. PMID 12766152.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Brill LM, Salomon AR, Ficarro SB, et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
• Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
• Zhang Y, Wolf-Yadlin A, Ross PL, et al. (2005). "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules". Mol. Cell. Proteomics. 4 (9): 1240–50. doi:10.1074/mcp.M500089-MCP200. PMID 15951569.
• Nishimura T, Yamaguchi T, Tokunaga A, et al. (2006). "Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2". Mol. Biol. Cell. 17 (3): 1273–85. doi:10.1091/mbc.E05-07-0700. PMC 1382316. PMID 16394100.
• Seifert M, Ampofo C, Mehraein Y, et al. (2007). "Expression analysis of human intersectin 2 gene (ITSN2) minor splice variants showing differential expression in normal human brain". Oncol. Rep. 17 (5): 1207–11. doi:10.3892/or.17.5.1207. PMID 17390067.
• Lim CS, Seet BT, Ingham RJ, et al. (2007). "The K15 protein of Kaposi's sarcoma-associated herpesvirus recruits the endocytic regulator intersectin 2 through a selective SH3 domain interaction". Biochemistry. 46 (35): 9874–85. doi:10.1021/bi700357s. PMID 17696407.