ITIH2

Inter-alpha-trypsin inhibitor heavy chain H2 is a protein that in humans is encoded by the ITIH2 gene.[5][6][7]

ITIH2
Identifiers
Aliases	ITIH2, H2P, SHAP, inter-alpha-trypsin inhibitor heavy chain 2
External IDs	OMIM: 146640 MGI: 96619 HomoloGene: 1668 GeneCards: ITIH2
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10p14 Start 7,703,316 bp[1] End 7,749,520 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2 A1|2 6.89 cM Start 10,094,593 bp[2] End 10,131,396 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • peptidase inhibitor activity • endopeptidase inhibitor activity • serine-type endopeptidase inhibitor activity Cellular component • blood microparticle • extracellular exosome • extracellular region • endoplasmic reticulum lumen • collagen-containing extracellular matrix Biological process • hyaluronan metabolic process • negative regulation of peptidase activity • negative regulation of endopeptidase activity • post-translational modification • cellular protein metabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	3698	16425
Ensembl	ENSG00000151655	ENSMUSG00000037254
UniProt	P19823	Q61703
RefSeq (mRNA)	NM_002216	NM_010582
RefSeq (protein)	NP_002207	NP_034712
Location (UCSC)	Chr 10: 7.7 – 7.75 Mb	Chr 2: 10.09 – 10.13 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

It is known to contain a Gla domain, and thus be dependent for production on post translational modification requiring vitamin K. Its function is also presumably dependent on calcium ions.

See also

• Inter-alpha-trypsin inhibitor
• ITIH1
• ITIH3
• ITIH4

References

1. GRCh38: Ensembl release 89: ENSG00000151655 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000037254 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Salier JP, Simon D, Rouet P, Raguenez G, Muscatelli F, Gebhard W, Guenet JL, Mattei MG (Dec 1992). "Homologous chromosomal locations of the four genes for inter-alpha-inhibitor and pre-alpha-inhibitor family in human and mouse: assignment of the ancestral gene for the lipocalin superfamily". Genomics. 14 (1): 83–8. doi:10.1016/S0888-7543(05)80287-3. PMID 1385302.
6. Jean L, Risler JL, Nagase T, Coulouarn C, Nomura N, Salier JP (May 1999). "The nuclear protein PH5P of the inter-alpha-inhibitor superfamily: a missing link between poly(ADP-ribose)polymerase and the inter-alpha-inhibitor family and a novel actor of DNA repair?". FEBS Lett. 446 (1): 6–8. doi:10.1016/S0014-5793(99)00173-8. PMID 10100603.
7. "Entrez Gene: ITIH2 inter-alpha (globulin) inhibitor H2".

Further reading

• Malki N, Balduyck M, Maes P, et al. (1992). "The heavy chains of human plasma inter-alpha-trypsin inhibitor: their isolation, their identification by electrophoresis and partial sequencing. Differential reactivity with concanavalin A.". Biol. Chem. Hoppe-Seyler. 373 (10): 1009–18. doi:10.1515/bchm3.1992.373.2.1009. PMID 1384548.
• Salier JP, Diarra-Mehrpour M, Sesboue R, et al. (1988). "Isolation and characterization of cDNAs encoding the heavy chain of human inter-alpha-trypsin inhibitor (I alpha TI): unambiguous evidence for multipolypeptide chain structure of I alpha TI". Proc. Natl. Acad. Sci. U.S.A. 84 (23): 8272–6. doi:10.1073/pnas.84.23.8272. PMC 299524. PMID 2446322.
• Gebhard W, Schreitmüller T, Hochstrasser K, Wachter E (1988). "Complementary DNA and derived amino acid sequence of the precursor of one of the three protein components of the inter-alpha-trypsin inhibitor complex". FEBS Lett. 229 (1): 63–7. doi:10.1016/0014-5793(88)80798-1. PMID 2450046.
• Salier JP, Diarra-Mehrpour M, Sesboüé R, et al. (1989). "Human inter-alpha-trypsin inhibitor. Isolation and characterization of heavy (H) chain cDNA clones coding for a 383 amino-acid sequence of the H chain". Biol. Chem. Hoppe-Seyler. 369 Suppl: 15–8. PMID 2462430.
• Diarra-Mehrpour M, Bourguignon J, Sesboüé R, et al. (1989). "Human plasma inter-alpha-trypsin inhibitor is encoded by four genes on three chromosomes". Eur. J. Biochem. 179 (1): 147–54. doi:10.1111/j.1432-1033.1989.tb14532.x. PMID 2465147.
• Enghild JJ, Thøgersen IB, Pizzo SV, Salvesen G (1989). "Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan". J. Biol. Chem. 264 (27): 15975–81. PMID 2476436.
• Schreitmüller T, Hochstrasser K, Reisinger PW, et al. (1987). "cDNA cloning of human inter-alpha-trypsin inhibitor discloses three different proteins". Biol. Chem. Hoppe-Seyler. 368 (8): 963–70. doi:10.1515/bchm3.1987.368.2.963. PMID 3663330.
• Huang L, Yoneda M, Kimata K (1994). "A serum-derived hyaluronan-associated protein (SHAP) is the heavy chain of the inter alpha-trypsin inhibitor". J. Biol. Chem. 268 (35): 26725–30. PMID 7504674.
• Morelle W, Capon C, Balduyck M, et al. (1994). "Chondroitin sulphate covalently cross-links the three polypeptide chains of inter-alpha-trypsin inhibitor". Eur. J. Biochem. 221 (2): 881–8. doi:10.1111/j.1432-1033.1994.tb18803.x. PMID 7513643.
• Wisniewski HG, Burgess WH, Oppenheim JD, Vilcek J (1994). "TSG-6, an arthritis-associated hyaluronan binding protein, forms a stable complex with the serum protein inter-alpha-inhibitor". Biochemistry. 33 (23): 7423–9. doi:10.1021/bi00189a049. PMID 7516184.
• Enghild JJ, Salvesen G, Thøgersen IB, et al. (1993). "Presence of the protein-glycosaminoglycan-protein covalent cross-link in the inter-alpha-inhibitor-related proteinase inhibitor heavy chain 2/bikunin". J. Biol. Chem. 268 (12): 8711–6. PMID 7682553.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Mizon C, Balduyck M, Albani D, et al. (1996). "Development of an enzyme-linked immunosorbent assay for human plasma inter-alpha-trypsin inhibitor (ITI) using specific antibodies against each of the H1 and H2 heavy chains". J. Immunol. Methods. 190 (1): 61–70. doi:10.1016/0022-1759(95)00257-X. PMID 8601712.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Olsen EH, Rahbek-Nielsen H, Thogersen IB, et al. (1998). "Posttranslational modifications of human inter-alpha-inhibitor: identification of glycans and disulfide bridges in heavy chains 1 and 2". Biochemistry. 37 (1): 408–16. doi:10.1021/bi971137d. PMID 9425062.
• Mizushima S, Nii A, Kato K, Uemura A (1998). "Gene expression of the two heavy chains and one light chain forming the inter-alpha-trypsin-inhibitor in human tissues". Biol. Pharm. Bull. 21 (2): 167–9. doi:10.1248/bpb.21.167. PMID 9514613.
• Flahaut C, Capon C, Balduyck M, et al. (1998). "Glycosylation pattern of human inter-alpha-inhibitor heavy chains". Biochem. J. 333 (3): 749–56. doi:10.1042/bj3330749. PMC 1219641. PMID 9677337.
• Hamm A, Veeck J, Bektas N, et al. (2008). "Frequent expression loss of Inter-alpha-trypsin inhibitor heavy chain (ITIH) genes in multiple human solid tumors: a systematic expression analysis". BMC Cancer. 8 (25): 25. doi:10.1186/1471-2407-8-25. PMC 2268946. PMID 18226209.