Folin's reagent

Folin's reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used as a derivatizing agent to measure levels of amines and amino acids.[1] The reagent reacts with them in alkaline solution to produce a fluorescent material that can be easily detected.[2]

Folin's reagent

Names
IUPAC name Sodium 3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate
Identifiers
CAS Number	521-24-4^Y
3D model (JSmol)	Interactive image
ChemSpider	10190
ECHA InfoCard	100.007.555
EC Number	208-308-9
PubChem CID	10636
UNII	T7185HP5SH^Y
CompTox Dashboard (EPA)	DTXSID8060167
InChI InChI=1S/C10H6O5S.Na/c11-8-5-9(16(13,14)15)6-3-1-2-4-7(6)10(8)12;/h1-5H,(H,13,14,15);/q;+1/p-1 Key: UBLXEEBHYISRFM-UHFFFAOYSA-M InChI=1/C10H6O5S.Na/c11-8-5-9(16(13,14)15)6-3-1-2-4-7(6)10(8)12;/h1-5H,(H,13,14,15);/q;+1/p-1 Key: UBLXEEBHYISRFM-REWHXWOFAQ
SMILES C1=CC=C2C(=C1)C(=CC(=O)C2=O)S(=O)(=O)[O-].[Na+]
Properties
Chemical formula	C10H5NaO5S
Molar mass	260.20 g/mol
Hazards
Safety data sheet	Oxford MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Y verify (what is ^YN ?)
Infobox references

This should not be confused with Folin-Ciocalteu reagent, that is used to detect phenolic compounds.

The Folin reagent can be used with an acidic secondary reagent to distinguish MDMA and related compounds from PMMA and related compounds.[3]

Substance	Reaction
MDMA	Pink[3]
MDE	No Reaction[3]
MDA	Yellow[3]
BZP	Orange to red[3]
TFMPP	Pinkish red[3]
Methylone	No reaction[3]
Methamphetamine	Pink[3]
Amphetamine	Light orange[3]
PMA	Light Yellow[3]
PMMA	Light orange[3]
Ketamine	No reaction[3]
Mescaline	No reaction[3]
Cocaine	No reaction[3]
Aspirin	No reaction[3]
Sugar	No reaction[3]

See also

• Pill testing
• Sullivan reaction
• Dille–Koppanyi reagent
• Froehde reagent
• Liebermann reagent
• Mandelin reagent
• Marquis reagent
• Mecke reagent
• Simon's reagent
• Zwikker reagent

References

1. Saurina J, Hernández-Cassou S (1994). "Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate". Journal of Chromatography A. 676 (2): 311–9. doi:10.1016/0021-9673(94)80431-1. PMID 7921184.
2. Kobayashi Y, Kubo H, Kinoshita T (1987). "Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography". Anal. Biochem. 160 (2): 392–8. doi:10.1016/0003-2697(87)90066-2. PMID 3578768.
3. "Folin Reagent Testing Kit". Dancesafe. June 2015. Archived from the original on 11 September 2015. Retrieved 8 August 2015.