FKBP7

FK506 binding protein 7 is a protein that in humans is encoded by the FKBP7 gene.[1] The gene is also known as FKBP23 and PPIase.[1] FKBP7 belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. Members of this family exhibit PPIase activity and function as molecular chaperones. The orthologous protein in mouse is located in the endoplasmic reticulum and binds calcium.[1][2]

Model organisms

Model organisms have been used in the study of FKBP7 function. A conditional knockout mouse line, called Fkbp7^{tm2a(KOMP)Wtsi}[3][4] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[5][6][7]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[8][9]

Fkbp7 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Brain histopathology	Normal
Salmonella infection	Normal[10]
Citrobacter infection	Normal[11]
All tests and analysis from[8][12]

Twenty three tests were carried out on mutant mice, but no significant abnormalities were observed.[8]

References

1. "FK506 binding protein 7". Retrieved 2011-12-06.
2. Nakamura, T.; Yabe, D.; Kanazawa, N.; Tashiro, K.; Sasayama, S.; Honjo, T. (1998). "Molecular Cloning, Characterization, and Chromosomal Localization of FKBP23, a Novel FK506-Binding Protein with Ca2+-Binding Ability". Genomics. 54 (1): 89–98. doi:10.1006/geno.1998.5571. PMID 9806833.
3. "International Knockout Mouse Consortium".
4. "Mouse Genome Informatics".
5. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
6. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
7. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
8. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
9. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
10. "Salmonella infection data for Fkbp7". Wellcome Trust Sanger Institute.
11. "Citrobacter infection data for Fkbp7". Wellcome Trust Sanger Institute.
12. Mouse Resources Portal, Wellcome Trust Sanger Institute.

Further reading

• Patterson, C. E.; Gao, J.; Rooney, A. P.; Davis, E. C. (2002). "Genomic Organization of Mouse and Human 65 kDa FK506-Binding Protein Genes and Evolution of the FKBP Multigene Family". Genomics. 79 (6): 881–889. doi:10.1006/geno.2002.6777. PMID 12036304.
• Matsuda, M.; Koide, T.; Yorihuzi, T.; Hosokawa, N.; Nagata, K. (2001). "Molecular Cloning of a Novel Ubiquitin-like Protein, UBIN, That Binds to ER Targeting Signal Sequences". Biochemical and Biophysical Research Communications. 280 (2): 535–540. doi:10.1006/bbrc.2000.4149. PMID 11162551.