FAM167A

Family with sequence similarity 167, member A is a protein in humans that is encoded by the FAM167A gene located on chromosome 8.[5] FAM167A and its paralogs are protein encoding genes containing the conserved domain DUF3259, a protein of unknown function.[6] FAM167A has many orthologs in which the domain of unknown function is highly conserved.

FAM167A
Identifiers
AliasesFAM167A, C8orf13, D8S265, family with sequence similarity 167 member A, DIORA-1
External IDsOMIM: 610085 MGI: 3606565 HomoloGene: 14243 GeneCards: FAM167A
Gene location (Human)
Chr.Chromosome 8 (human)[1]
Band8p23.1Start11,421,476 bp[1]
End11,475,908 bp[1]
Orthologs
SpeciesHumanMouse
Entrez

83648

219148

Ensembl

ENSG00000154319

ENSMUSG00000035095

UniProt

Q96KS9

Q6P1G6

RefSeq (mRNA)

NM_053279

NM_177628

RefSeq (protein)

NP_444509

NP_808296

Location (UCSC)Chr 8: 11.42 – 11.48 MbChr 14: 63.44 – 63.47 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Gene

Locus

On chromosome 8, FAM167A is positioned between c8orf12 (anti-sense) and BLK (anti-sense).[7] The exact locus of FAM167A is 8p23-22 and spans from 11,278,972 to 11,332,224, a total of 53,253 base pairs. The promoter spans from 11324145 to 11324476 on the negative strand, thereby the first basepair is actually on 11324476. There are no human isoforms found.

Aliases

Family with Sequence Similarity 167, Member A is also known as FAM167A, c8orf13, or D8S265.[8]

Homology

Paralogs

FAM167A has one paralog, FAM167B also known as c1orf90.[9] FAM167B is located at 1p35.1 on the plus strand and is composed of 163 amino acids and also contains DUF3259.[10]

Orthologs
A chart showing divergence and sequence identity

FAM167A has orthologs in 82 organisms and is conserved across chimpanzees, dog, cow, mouse, chicken, rat, frogs, and zebrafish.[11][12]

SpeciesSpecies Common NameNCBI Accession Number (Protein)Amino Acid LengthProtein IdentityDivergence date from Humans (million years ago)
Homo SapiensHumanNP_444509214100%0
Pan TroglodytesChimpanzeeXP_00113912221499%6.3
Macaca FascicularisMacaqueXP_005562638.121496%29
Neterocephalus GlaberNaked mole ratXP_00484850921484%92.3
Felis CatusCatXP_00398489020980%94.2
Equus CaballusHorseXP_00149796820380%94.2
Alligator SinensisChinese AlligatorXP_00602821521170%296
Anolis CarolinensisCarolina AnoleXP_00322798421564%296
Danio RerioZebrafishNP_102072120459%400.1
Latimeria ChalumnaeAfrican CoelacanthXP_0599457014843%414.9
Ciona IntestinalisSea SquirtXP_00212342125527%722.5

As shown in the table above, FAM167A is highly conserved across many orthologs of various divergence dates. The exact degree of conservation follows what is expected due to the evolutionary track of a protein.

Protein

Primary sequence

The gene that encodes FAM167A is 214 amino acids in length. The molecular weight in humans of the FAM167A protein is 24.2 kdal and the isoelectric point is measured to be 5.887 in Homo sapiens.[13] Mouse and chicken orthologs were shown to have a molecular weight of ± 0.5 kdal and isoelectric points were ±0.6.

Variants
Alternative splices of FAM167A

As per the results on AceView, shown right, the FAM167A gene contains 13 introns. The gene is also "well expressed" at 1.2 times the average gene. Transcription produces 9 different mRNAs, 8 of which are alternatively spliced and 1 unspliced form. 4 of the spliced proteins, which includes 2 isoforms, are considered to be good while the remaining five are partial or not good proteins.[14]

Secondary structure

FAM167A has a leucine zipper as part of its secondary structure as noted by the four heptad leucine repeat regions shown in SAPS. The leucine zipper is a portion of the DUF domain. Predictions of the secondary structure for the FAM167A protein are mostly that it is made of alpha helices and coiled coils, which would be reasonable as there is a coiled coil domain. The C-terminus end of DUF3259 is generally agreed upon in the PELE program to be a region of potential beta sheets and coiled coils. Using PELE, there is some consensus amongst the eight different outputs given as to the general secondary structure of the protein. There are no transmembrane domains as predicted on the FAM167A protein.

Interacting proteins
Web of interactions between FAM167A and other proteins

Using the MINT, STRING, and IntAct tools on Genecards, the sources have a consensus on the interactions between FAM167A and BANK1 as well as the BLK gene.[15] These proteins are already known to interact with FAM167A in the development of several diseases such as Sjogren's disease and systemic sclerosis. In both the case of BANK1 and BLK, there is literature to back up the possible connections and interactions between the two proteins in disease development.

Post-translational modification

No glycosylation sites have been found, as searched using tools on Expasy.org. There was a site for serine phosphorylation on both the human and mouse proteins and two for tyrosine phosphorylation, amino acids 147, 159, and 170 respectfully. Phosphorylation sites are used for various regulatory functions such as enzyme inhibition, protein-protein interactions, and protein degradation.

Function
Human tissue expression

Micro arrays show that FAM167A has varied expression in reactions to cancers, but no information regarding the exact function of FAM167A can be drawn from these micro arrays. FAM167A has ubiquitously low expression in all tissues types throughout the body.[16] In mouse it has a higher expression in the skin, B-cells, and spleen, but the same low expression in all other cell types.[17]

Clinical significance

SNPs in the regions between FAM167A and the BLK gene have been associated with the development of Sjogren's syndrome in a Han Chinese population,[18] as well as in a Scandinavian population.[19] The FAM167A-BLK region has also been linked to systemic sclerosis by comparing functional variants in the C8orf13-BLK locus in a Caucasian population. Results of the study confirms the C8orf13-BLK locus as a systemic sclerosis risk locus, strongest effects were observed in the interactions between that locus and BANK1.[20]

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References
  1. GRCh38: Ensembl release 89: ENSG00000154319 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000035095 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. GeneCard for FAM167A
  6. Marchler-Bauer A et al. (2013) (2013). "CDD: conserved domains and protein three-dimensional structure". Nucleic Acids Res. 41. D1:D384-52. doi:10.1093/nar/gks1243. PMC 3531192. PMID 23197659.
  7. https://www.ncbi.nlm.nih.gov/epigenomics/view/genome/83648/?term=FAM167A
  8. National Human Genome Research Institute. "FAM167A Symbol Report".
  9. GeneCard for FAM167A
  10. "Protein FAM167B [Homo sapiens] - Protein - NCBI".
  11. "Protein FAM167A [Homo sapiens] - Protein - NCBI".
  12. "FAM167A family with sequence similarity 167 member A [Homo sapiens (human)] - Gene - NCBI".
  13. Brendel V, Bucher P, Nourbakhsh IR, Blaisdell BE, Karlin S (March 1992). "Methods and algorithms for statistical analysis of protein sequences". Proceedings of the National Academy of Sciences of the United States of America. 89 (6): 2002–6. Bibcode:1992PNAS...89.2002B. doi:10.1073/pnas.89.6.2002. PMC 48584. PMID 1549558.
  14. AceView NCBI. "Homo sapiens gene FAM167A, encoding family with sequence similarity 167, member A."
  15. STRING. "Evidence view of FAM167A interacting genes".
  16. BioGPS. "SymAtlas Human tissue expression".
  17. BioGPS. "Tissue expression in house mouse".
  18. Sun F, Xu J, Wu Z, Li P, Chen H, Su J, You X, Li M, Zhao Y, Tian X, Li Y, Zhang F (2013). "Polymorphisms in the FAM167A-BLK, but not BANK1, are associated with primary Sjögren's syndrome in a Han Chinese population". Clinical and Experimental Rheumatology. 31 (5): 704–10. PMID 23899688.
  19. Nordmark G, Kristjansdottir G, Theander E, Appel S, Eriksson P, Vasaitis L, Kvarnström M, Delaleu N, Lundmark P, Lundmark A, Sjöwall C, Brun JG, Jonsson MV, Harboe E, Gøransson LG, Johnsen SJ, Söderkvist P, Eloranta ML, Alm G, Baecklund E, Wahren-Herlenius M, Omdal R, Rönnblom L, Jonsson R, Syvänen AC (March 2011). "Association of EBF1, FAM167A(C8orf13)-BLK and TNFSF4 gene variants with primary Sjögren's syndrome". Genes and Immunity. 12 (2): 100–9. doi:10.1038/gene.2010.44. PMID 20861858.
  20. Coustet B, Dieudé P, Guedj M, Bouaziz M, Avouac J, Ruiz B, Hachulla E, Diot E, Cracowski JL, Tiev K, Sibilia J, Mouthon L, Frances C, Amoura Z, Carpentier P, Cosnes A, Meyer O, Kahan A, Boileau C, Chiocchia G, Allanore Y (July 2011). "C8orf13-BLK is a genetic risk locus for systemic sclerosis and has additive effects with BANK1: results from a large french cohort and meta-analysis". Arthritis and Rheumatism. 63 (7): 2091–6. doi:10.1002/art.30379. PMID 21480188.
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