EME1

Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene.[5][6] It forms a complex with MUS81 which resolves Holliday junctions.

EME1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2ZIU, 2ZIV, 2ZIW, 2ZIX, 4P0P, 4P0Q, 4P0R, 4P0S
Identifiers
Aliases	EME1, MMS4L, SLX2A, essential meiotic structure-specific endonuclease 1
External IDs	OMIM: 610885 MGI: 3576783 HomoloGene: 16123 GeneCards: EME1
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17q21.33 Start 50,373,220 bp[1] End 50,381,483 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11|11 D Start 94,644,996 bp[2] End 94,653,964 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • endonuclease activity • nuclease activity • GO:0001948 protein binding • hydrolase activity • metal ion binding • endodeoxyribonuclease activity • crossover junction endodeoxyribonuclease activity Cellular component • nuclear heterochromatin • nucleolus • nucleoplasm • nuclear chromatin • cell nucleus • Holliday junction resolvase complex Biological process • cellular response to DNA damage stimulus • DNA recombination • interstrand cross-link repair • nucleic acid phosphodiester bond hydrolysis • response to intra-S DNA damage checkpoint signaling • DNA repair • resolution of meiotic recombination intermediates • double-strand break repair • replication fork processing • intra-S DNA damage checkpoint Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	146956	268465
Ensembl	ENSG00000154920	ENSMUSG00000039055
UniProt	Q96AY2	Q8BJW7
RefSeq (mRNA)	NM_001166131 NM_152463	NM_177752 NM_001379688
RefSeq (protein)	NP_001159603 NP_689676	NP_808420 NP_001366617
Location (UCSC)	Chr 17: 50.37 – 50.38 Mb	Chr 11: 94.64 – 94.65 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000154920 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000039055 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ciccia A, Constantinou A, West SC (Jun 2003). "Identification and characterization of the human mus81-eme1 endonuclease". J Biol Chem. 278 (27): 25172–8. doi:10.1074/jbc.M302882200. PMID 12721304.
6. "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)".

Further reading

• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Oğrünç M, Sancar A (2003). "Identification and characterization of human MUS81-MMS4 structure-specific endonuclease". J. Biol. Chem. 278 (24): 21715–20. doi:10.1074/jbc.M302484200. PMID 12686547.
• Abraham J, Lemmers B, Hande MP, et al. (2004). "Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells". EMBO J. 22 (22): 6137–47. doi:10.1093/emboj/cdg580. PMC 275438. PMID 14609959.
• Blais V, Gao H, Elwell CA, et al. (2004). "RNA Interference Inhibition of Mus81 Reduces Mitotic Recombination in Human Cells". Mol. Biol. Cell. 15 (2): 552–62. doi:10.1091/mbc.E03-08-0580. PMC 329235. PMID 14617801.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Hiyama T, Katsura M, Yoshihara T, et al. (2006). "Haploinsufficiency of the Mus81–Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells". Nucleic Acids Res. 34 (3): 880–92. doi:10.1093/nar/gkj495. PMC 1360746. PMID 16456034.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.