EIF4E3

Eukaryotic translation initiation factor 4E family member 3 is a protein that in humans is encoded by the EIF4E3 gene.[5]

EIF4E3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4B6U, 4B6V
Identifiers
Aliases	EIF4E3, eIF-4E3, eIF4E-3, eukaryotic translation initiation factor 4E family member 3
External IDs	OMIM: 609896 MGI: 1914142 HomoloGene: 41652 GeneCards: EIF4E3
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p13 Start 71,675,414 bp[1] End 71,754,773 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6|6 D3 Start 99,625,135 bp[2] End 99,666,771 bp[2]
Gene ontology Molecular function • translation initiation factor activity • RNA binding • RNA 7-methylguanosine cap binding Cellular component • cytoplasm • cytosol • mRNA cap binding complex • eukaryotic translation initiation factor 4F complex Biological process • translational initiation • regulation of translation • protein biosynthesis Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	317649	66892
Ensembl	ENSG00000163412	ENSMUSG00000093661
UniProt	Q8N5X7	Q9DBB5
RefSeq (mRNA)	NM_001134649 NM_001134650 NM_001134651 NM_001282886 NM_173359	NM_025829
RefSeq (protein)	NP_001128121 NP_001128122 NP_001128123 NP_001269815 NP_775495	NP_080105
Location (UCSC)	Chr 3: 71.68 – 71.75 Mb	Chr 6: 99.63 – 99.67 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome.[5][6]

Model organisms

Eif4e3 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Skin Histopathology	Normal
Salmonella infection	Normal[7]
Citrobacter infection	Normal[8]
All tests and analysis from[9][10]

Model organisms have been used in the study of EIF4E3 function. A conditional knockout mouse line, called Eif4e3^{tm1a(KOMP)Wtsi}[11][12] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[13][14][15]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][16] Twenty four tests were carried out on mutant mice but no significant abnormalities were observed.[9]

References

1. GRCh38: Ensembl release 89: ENSG00000163412 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000093661 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: Eukaryotic translation initiation factor 4E family member 3". Retrieved 2011-09-28.
6. Joshi, B.; Cameron, A.; Jagus, R. (2004). "Characterization of mammalian eIF4E-family members". European Journal of Biochemistry. 271 (11): 2189–2203. doi:10.1111/j.1432-1033.2004.04149.x. PMID 15153109.
7. "Salmonella infection data for Eif4e3". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Eif4e3". Wellcome Trust Sanger Institute.
9. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248): 0. doi:10.1111/j.1755-3768.2010.4142.x.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
14. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
15. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
16. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

External links

• Overview of all the structural information available in the PDB for UniProt: Q9DBB5 (Mouse Eukaryotic translation initiation factor 4E type 3) at the PDBe-KB.