DBF4

Protein DBF4 homolog A is a protein that is encoded by the DBF4 gene in humans.[5][6][7]

DBF4
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4F99, 4F9A, 4F9B, 4F9C
Identifiers
Aliases	DBF4, ASK, CHIF, DBF4A, ZDBF1, DBF4 zinc finger
External IDs	OMIM: 604281 MGI: 1351328 HomoloGene: 40892 GeneCards: DBF4
Gene location (Human) Chr. Chromosome 7 (human)[1] Band 7q21.12 Start 87,876,216 bp[1] End 87,909,553 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 A1 Start 8,396,973 bp[2] End 8,422,716 bp[2]
Gene ontology Molecular function • zinc ion binding • GO:0010577 enzyme activator activity • metal ion binding • nucleic acid binding • GO:0001948 protein binding • protein kinase binding • protein serine/threonine kinase activator activity Cellular component • nucleoplasm • cell nucleus • nuclear body • Dbf4-dependent protein kinase complex Biological process • DNA replication • cell cycle • GO:0048554 positive regulation of catalytic activity • G1/S transition of mitotic cell cycle • positive regulation of DNA replication • positive regulation of protein serine/threonine kinase activity • positive regulation of nuclear cell cycle DNA replication • regulation of cell cycle phase transition Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10926	27214
Ensembl	ENSG00000006634	ENSMUSG00000002297
UniProt	Q9UBU7	Q9QZ41
RefSeq (mRNA)	NM_006716 NM_001318060 NM_001318061 NM_001318062	NM_001190717 NM_013726
RefSeq (protein)	NP_001304989 NP_001304990 NP_001304991 NP_006707	NP_001177646 NP_038754
Location (UCSC)	Chr 7: 87.88 – 87.91 Mb	Chr 5: 8.4 – 8.42 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

DBF4 has been shown to interact with:

• Cell division cycle 7-related protein kinase,[5][8][9]
• MCM3,[8]
• MCM7,[8]
• ORC2L,[8] and
• ORC6L.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000006634 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000002297 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Kumagai H, Sato N, Yamada M, Mahony D, Seghezzi W, Lees E, Arai K, Masai H (Jul 1999). "A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells". Molecular and Cellular Biology. 19 (7): 5083–95. doi:10.1128/MCB.19.7.5083. PMC 84351. PMID 10373557.
6. Lepke M, Pütter V, Staib C, Kneissl M, Berger C, Hoehn K, Nanda I, Schmid M, Grummt F (Sep 1999). "Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes". Molecular & General Genetics. 262 (2): 220–9. doi:10.1007/s004380051078. PMID 10517317.
7. "Entrez Gene: DBF4 DBF4 homolog (S. cerevisiae)".
8. Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
9. Jiang W, McDonald D, Hope TJ, Hunter T (Oct 1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication". The EMBO Journal. 18 (20): 5703–13. doi:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313.

Further reading

• Jiang W, McDonald D, Hope TJ, Hunter T (Oct 1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication". The EMBO Journal. 18 (20): 5703–13. doi:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313.
• Yamada M, Sato N, Taniyama C, Ohtani K, Arai K, Masai H (Aug 2002). "A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase". The Journal of Biological Chemistry. 277 (31): 27668–81. doi:10.1074/jbc.M202884200. PMID 12015319.
• Wu X, Lee H (Nov 2002). "Human Dbf4/ASK promoter is activated through the Sp1 and MluI cell-cycle box (MCB) transcription elements". Oncogene. 21 (51): 7786–96. doi:10.1038/sj.onc.1205914. PMID 12420215.
• Duncker BP, Shimada K, Tsai-Pflugfelder M, Pasero P, Gasser SM (Dec 2002). "An N-terminal domain of Dbf4p mediates interaction with both origin recognition complex (ORC) and Rad53p and can deregulate late origin firing". Proceedings of the National Academy of Sciences of the United States of America. 99 (25): 16087–92. doi:10.1073/pnas.252093999. PMC 138569. PMID 12441400.
• Costanzo V, Shechter D, Lupardus PJ, Cimprich KA, Gottesman M, Gautier J (Jan 2003). "An ATR- and Cdc7-dependent DNA damage checkpoint that inhibits initiation of DNA replication". Molecular Cell. 11 (1): 203–13. doi:10.1016/S1097-2765(02)00799-2. PMID 12535533.
• Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
• Lehner B, Sanderson CM (Jul 2004). "A protein interaction framework for human mRNA degradation". Genome Research. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Schnepp RW, Hou Z, Wang H, Petersen C, Silva A, Masai H, Hua X (Sep 2004). "Functional interaction between tumor suppressor menin and activator of S-phase kinase". Cancer Research. 64 (18): 6791–6. doi:10.1158/0008-5472.CAN-04-0724. PMID 15374998.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Gérard A, Koundrioukoff S, Ramillon V, Sergère JC, Mailand N, Quivy JP, Almouzni G (Aug 2006). "The replication kinase Cdc7-Dbf4 promotes the interaction of the p150 subunit of chromatin assembly factor 1 with proliferating cell nuclear antigen". EMBO Reports. 7 (8): 817–23. doi:10.1038/sj.embor.7400750. PMC 1525143. PMID 16826239.
• Tsuji T, Ficarro SB, Jiang W (Oct 2006). "Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells". Molecular Biology of the Cell. 17 (10): 4459–72. doi:10.1091/mbc.E06-03-0241. PMC 1635350. PMID 16899510.
• Heffernan TP, Unsal-Kaçmaz K, Heinloth AN, Simpson DA, Paules RS, Sancar A, Cordeiro-Stone M, Kaufmann WK (Mar 2007). "Cdc7-Dbf4 and the human S checkpoint response to UVC". The Journal of Biological Chemistry. 282 (13): 9458–68. doi:10.1074/jbc.M611292200. PMC 1839878. PMID 17276990.