C2C12

C2C12 is an immortalized mouse myoblast cell line. The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann Institute of Science in Israel in 1977.[1] Developed for in vitro studies of myoblasts isolated from the complex interactions of in vivo conditions, C2C12 cells are useful in biomedical research.[2] These cells are capable of rapid proliferation under high serum conditions and differentiation into myoblasts under low serum conditions. Mononucleated myoblasts can later fuse to form multinucleated myotubes under low serum conditions or starvation, leading to the precursors of contractile skeletal muscle cells in the process of myogenesis.[3] C2C12 cells are used to study the differentiation of myoblasts, osteoblasts, and myogenesis, to express various target proteins, and to explore mechanistic biochemical pathways.

C2C12 myotubes under light microscope, 10x magnification

Morphology

Wild-type C2C12 cells have a radial branching morphology consisting of long fibers extending in many directions. C2C12 cells can be cultured in a variety of conditions to induce specific responses of interest. For example, assisted by the cell line's high differentiation rate and fusion rate, fibronectin templates can be micro-plated to petri dishes or cell culture flasks in order to induce specific growth patterns, such as that of skeletal muscle cell interactions with extracellular matrix components.[4] The introduction of adhesion molecules can alter the growth pattern of C2C12 cells to a longitudinal distribution exhibiting polarity.[5] There are many ways to regulate the shape of C2C12 myoblasts genetically and environmentally, from stress, to cytoskeleton alteration, to growth factors. The scaffolding of C2C12 cells is particularly important for studying muscle tissue regeneration post-injury or after tissue wasting due to disease or ICU rehabilitation.

Uses in Research

C2C12 cells have been shown to effectively incorporate exogenous cDNA and nucleic acids by transfection. In the piloting research originally conducted by Yaffe and Saxel, C2C12 were obtained through serial passage of myoblasts cultured from the thigh muscle of C3H mice after crush injury. In their study, a set of C2C12 cells were cultured from mice homozygous recessive for the dy gene. These mice exhibited a syndrome similar to early-onset human muscular dystrophy. Normal mouse myoblasts were cultured from 2-month old C3H mice after crush injury. Within two days, the normal cells differentiated into spindle-shaped mononucleated myoblasts. After four days, multinucleated myotube networks formed, and a few days after, sarcomeres and Z-lines could be observed.[6] In contrast, the dystrophic cells formed shortened fibers covered in fibroblasts, a hallmark of muscle wasting.[1]

C2C12 cells demonstrate rapid development and maturation into functional skeletal muscle cells or cardiac muscle cells, having the ability to contract and generate force.[6] The rate of muscle formation from C2C12 cells can be controlled by the introduction of loss-of-functions genes vital for the fusion of myoblasts and myogenesis.[7] Under necrotic conditions, such as tumor necrosis factor alpha (TNF-α), direct protein loss, particularly myosin heavy chain protein, in C2C12 skeletal muscle cells has been shown.[8] C2C12 cells were used to elucidate inactivated X chromosome (Xi) replication during early S-phase of the cell cycle and is regulated epigenetically.[9] C2C12 cells are especially convenient for studying the cell cycle due to its high division rate.

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References
  1. Yaffe, David; Saxel, Ora (1977-12-22). "Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle". Nature. 270 (5639): 725–727. Bibcode:1977Natur.270..725Y. doi:10.1038/270725a0. ISSN 0028-0836. PMID 563524.
  2. "C2C12 Cell Line". Retrieved 12 July 2018.
  3. "Working with the C2C12 cell line". Research in Myogenesis. 2012-02-04. Retrieved 2017-05-03.
  4. Bajaj, Piyush; Reddy, Bobby; Millet, Larry; Wei, Chunan; Zorlutuna, Pinar; Bao, Gang; Bashir, Rashid (2011-09-01). "Patterning the differentiation of C2C12 skeletal myoblasts". Integrative Biology. 3 (9): 897–909. doi:10.1039/c1ib00058f. ISSN 1757-9708. PMID 21842084.
  5. Mermelstein, C.S. (May 5, 2003). "Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation" (PDF). Brazilian Journal of Medical and Biological Research. 36 (8): 1111–1116. doi:10.1590/s0100-879x2003000800018. PMID 12886466.
  6. McMahon, D. K.; Anderson, P. A.; Nassar, R.; Bunting, J. B.; Saba, Z.; Oakeley, A. E.; Malouf, N. N. (1994-06-01). "C2C12 cells: biophysical, biochemical, and immunocytochemical properties". American Journal of Physiology. Cell Physiology. 266 (6): C1795–C1802. doi:10.1152/ajpcell.1994.266.6.c1795. ISSN 0363-6143. PMID 8023908.
  7. Bi, Pengpeng; Ramirez-Martinez, Andres; Li, Hui; Cannavino, Jessica; McAnally, John R.; Shelton, John M.; Sánchez-Ortiz, Efrain; Bassel-Duby, Rhonda; Olson, Eric N. (2017-04-21). "Control of muscle formation by the fusogenic micropeptide myomixer". Science. 356 (6335): 323–327. Bibcode:2017Sci...356..323B. doi:10.1126/science.aam9361. ISSN 1095-9203. PMC 5502127. PMID 28386024.
  8. Li, Y. P.; Schwartz, R. J.; Waddell, I. D.; Holloway, B. R.; Reid, M. B. (1998-07-01). "Skeletal muscle myocytes undergo protein loss and reactive oxygen-mediated NF-kappaB activation in response to tumor necrosis factor alpha". FASEB Journal. 12 (10): 871–880. doi:10.1096/fasebj.12.10.871. ISSN 0892-6638. PMID 9657527.
  9. Casas-Delucchi, Corella S.; Brero, Alessandro; Rahn, Hans-Peter; Solovei, Irina; Wutz, Anton; Cremer, Thomas; Leonhardt, Heinrich; Cardoso, M. Cristina (2011-03-01). "Histone acetylation controls the inactive X chromosome replication dynamics". Nature Communications. 2: 222. Bibcode:2011NatCo...2..222C. doi:10.1038/ncomms1218. ISSN 2041-1723. PMC 3072080. PMID 21364561.
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