Bisbenzimide
Bisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications.[1] Several related chemical compounds are used for similar purposes and are collectively called Hoechst stains.
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| IUPAC name
2′-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-1H,3′H-2,5′-bibenzimidazole | |
| Other names
Hoechst 33342; Hoe 33342 | |
| Identifiers | |
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3D model (JSmol) |
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| ChemSpider | |
| ECHA InfoCard | 100.041.523 |
PubChem CID |
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CompTox Dashboard (EPA) |
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| Properties | |
| C27H28N6O | |
| Molar mass | 452.562 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Application
Bisbenzimide tends to bind to adenine–thymine-rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.
Nuclei of Platynereis dumerilii larvae stained with Hoechst 33342
gollark: Oh yes, true, you need to have a large body of water.
gollark: The waste is not actually a problem since you can just bury it somewhere stable forever.
gollark: Meanwhile, nuclear can produce basically arbitrary amounts of power regardless of time of day with very little land requirement.
gollark: Wikipedia says that on average each bit of the Earth only gets about 400W/m², and they are not 75% efficient, so no.
gollark: They don't use all wavelengths, and they don't use what they do use entirely efficiently.
References
External links
- Fluorescence Spectra: http://www.fluorophores.tugraz.at/substance/463
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