Bis-tris propane

Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, also known as BTP, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pKa values which are close in value. This buffer is primarily used in biochemistry and molecular biology.

Bis-tris propane
Names
Other names
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxymethyl)propane-1,3-diol]
Identifiers
3D model (JSmol)
1786109
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard 100.058.981
EC Number
  • 264-899-3
1734507
MeSH 1,3-bis(tris(hydroxymethyl)methylamino)propane
Properties
C11H26N2O6
Molar mass 282.337 g·mol−1
Appearance White crystals
Melting point 164 to 165 °C (327 to 329 °F; 437 to 438 K)
log P −2.794
Related compounds
Related compounds
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Applications

A review of DNA polymerase fidelity cites bis-tris propane as a suitable buffer for polymerase chain reaction (PCR).[1] Bis-Tris propane has also been used with HCl buffer for stabilization of farnesyl diphosphate isolated from a strain of Saccharomyces cerevisiae.[2] It has also been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodospin.[3] Use of Bis-Tris propane has also been documented in an investigation of the MgATPase activity of the myosin subfragment 1 monomer.[4] The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane.[5] Bis-Tris propane wide buffering range is also useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria.[6]

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See also

References
  1. Eckert K.A. & Kunkel, T.A. (1991). "DNA polymerase fidelity and the polymerase chain reaction". PCR Methods Appl. 1 (1): 17–24. doi:10.1101/gr.1.1.17. PMID 1842916.
  2. Song, L. (2003). "Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants". Anal. Biochem. 317 (2): 180–185. doi:10.1016/S0003-2697(03)00138-6. PMID 12758256.
  3. Toth-Boconadi, R.; et al. (2000). "Buffer effects on electric signals of light-excited bacteriorhodospin". Biophys. J. 78 (6): 3170–3177. Bibcode:2000BpJ....78.3170T. doi:10.1016/S0006-3495(00)76853-6. PMC 1300898. PMID 10827993.
  4. Bachouchi N, Garrigos M, Morel JE (1986). "MgATPase activity of myosin subfragment 1. The dimer is more active than the monomer". J. Mol. Biol. 191 (2): 247–254. doi:10.1016/0022-2836(86)90261-5. PMID 2949083.
  5. Wenner, J.R. & Bloomfield, V.A. (1999). "Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage". Anal. Biochem. 268 (2): 201–212. doi:10.1006/abio.1998.3079. PMID 10075809.
  6. Ivannikov, M.; et al. (2013). "Mitochondrial Free Ca2+ Levels and Their Effects on Energy Metabolism in Drosophila Motor Nerve Terminals". Biophys. J. 104 (11): 2353–2361. Bibcode:2013BpJ...104.2353I. doi:10.1016/j.bpj.2013.03.064. PMC 3672877. PMID 23746507.
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