Baby hamster kidney cell

Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.

BHK and virus infection

BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain)[1]. BHK-21 cells are resistant to poliovirus 2 and Rabbit vesivirus (RaV). The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes.[2]

Utilization

The BHK-21 cells are useful for transformations and for stable and temporary transfections.[2] BHK cells are also used to study viral infections[1][3].

  • High-glucose DMEM
  • FBS fetal bovine serum 5% (when you freeze, add 10%)
  • GLU glutamine 1%
  • PSA regular antibiotics 1%
  • Splitting :10 by Trypsin[4]

References
  1. Timm C, Gupta A, Yin J (August 2015). "Robust kinetics of an RNA virus: Transcription rates are set by genome levels". Biotechnology and Bioengineering. 112 (8): 1655–62. doi:10.1002/bit.25578. PMC 5653219. PMID 25726926.
  2. "BHK-21 Cells". Retrieved 24 April 2018.
  3. Lim KI, Lang T, Lam V, Yin J (September 2006). "Model-based design of growth-attenuated viruses". PLoS Computational Biology. 2 (9): e116. Bibcode:2006PLSCB...2..116L. doi:10.1371/journal.pcbi.0020116. PMC 1557587. PMID 16948530.
  4. "Microscopy Resource Center | Olympus Life Science". www.olympusmicro.com.
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