ASAH2

Neutral ceramidase is an enzyme that in humans is encoded by the ASAH2 gene.[5][6]

ASAH2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4WGK
Identifiers
Aliases	ASAH2, BCDase, HNAC1, LCDase, N-CDase, NCDase, N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2, N-acylsphingosine amidohydrolase 2
External IDs	OMIM: 611202 MGI: 1859310 HomoloGene: 10310 GeneCards: ASAH2
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10q11.23 Start 50,182,778 bp[1] End 50,279,720 bp[1]
Gene location (Mouse) Chr. Chromosome 19 (mouse)[2] Band 19|19 C1 Start 31,982,597 bp[2] End 32,108,211 bp[2]
Gene ontology Molecular function • hydrolase activity • ceramidase activity • calcium ion binding • zinc ion binding • acylsphingosine deacylase activity • metal ion binding Cellular component • integral component of membrane • cell membrane • membrane • mitochondrion • extracellular region Biological process • lipid metabolism • sphingolipid metabolic process • apoptotic process • sphingosine metabolic process • ceramide metabolic process • response to organic substance • long-chain fatty acid biosynthetic process • sphingosine biosynthetic process • ceramide catabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	56624	54447
Ensembl	ENSG00000188611	ENSMUSG00000024887
UniProt	Q9NR71	Q9JHE3
RefSeq (mRNA)	NM_001143974 NM_019893	NM_018830
RefSeq (protein)	NP_001137446 NP_063946	NP_061300
Location (UCSC)	Chr 10: 50.18 – 50.28 Mb	Chr 19: 31.98 – 32.11 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000188611 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024887 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. El Bawab S, Roddy P, Qian T, Bielawska A, Lemasters JJ, Hannun YA (Aug 2000). "Molecular cloning and characterization of a human mitochondrial ceramidase". J Biol Chem. 275 (28): 21508–13. doi:10.1074/jbc.M002522200. PMID 10781606.
6. "Entrez Gene: ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2".

External links

• Human ASAH2 genome location and ASAH2 gene details page in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: Q9NR71 (Neutral ceramidase; ASAH2) at the PDBe-KB.

Further reading

• Matoba R, Okubo K, Hori N, et al. (1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10.1016/0378-1119(94)90293-3. PMID 8076819.
• Tani M, Okino N, Mori K, et al. (2000). "Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases". J. Biol. Chem. 275 (15): 11229–34. doi:10.1074/jbc.275.15.11229. PMID 10753931.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Choi MS, Anderson MA, Zhang Z, et al. (2004). "Neutral ceramidase gene: role in regulating ceramide-induced apoptosis". Gene. 315: 113–22. doi:10.1016/S0378-1119(03)00721-2. PMID 14557071.
• Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature. 429 (6990): 375–81. Bibcode:2004Natur.429..375D. doi:10.1038/nature02462. PMID 15164054.
• Monick MM, Mallampalli RK, Bradford M, et al. (2004). "Cooperative prosurvival activity by ERK and Akt in human alveolar macrophages is dependent on high levels of acid ceramidase activity". J. Immunol. 173 (1): 123–35. doi:10.4049/jimmunol.173.1.123. PMID 15210766.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Hwang YH, Tani M, Nakagawa T, et al. (2005). "Subcellular localization of human neutral ceramidase expressed in HEK293 cells". Biochem. Biophys. Res. Commun. 331 (1): 37–42. doi:10.1016/j.bbrc.2005.03.134. PMID 15845354.
• Osawa Y, Uchinami H, Bielawski J, et al. (2005). "Roles for C16-ceramide and sphingosine 1-phosphate in regulating hepatocyte apoptosis in response to tumor necrosis factor-alpha". J. Biol. Chem. 280 (30): 27879–87. doi:10.1074/jbc.M503002200. PMID 15946935.
• Tani M, Sano T, Ito M, Igarashi Y (2006). "Mechanisms of sphingosine and sphingosine 1-phosphate generation in human platelets". J. Lipid Res. 46 (11): 2458–67. doi:10.1194/jlr.M500268-JLR200. PMID 16061940.
• Galadari S, Wu BX, Mao C, et al. (2006). "Identification of a novel amidase motif in neutral ceramidase". Biochem. J. 393 (Pt 3): 687–95. doi:10.1042/BJ20050682. PMC 1360721. PMID 16229686.
• Xu R, Jin J, Hu W, et al. (2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". FASEB J. 20 (11): 1813–25. doi:10.1096/fj.05-5689com. PMID 16940153.
• Avramopoulos D, Wang R, Valle D, et al. (2007). "A novel gene derived from a segmental duplication shows perturbed expression in Alzheimer's disease". Neurogenetics. 8 (2): 111–20. doi:10.1007/s10048-007-0081-5. PMID 17334805.
• Ohlsson L, Palmberg C, Duan RD, et al. (2007). "Purification and characterization of human intestinal neutral ceramidase". Biochimie. 89 (8): 950–60. doi:10.1016/j.biochi.2007.03.009. PMID 17475390.