API5

The human gene API5 encodes the protein Apoptosis inhibitor 5.[5][6]

API5
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3U0R, 3V6A
Identifiers
Aliases	API5, AAC-11, AAC11, apoptosis inhibitor 5
External IDs	OMIM: 609774 MGI: 1888993 HomoloGene: 4809 GeneCards: API5
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p12 Start 43,311,963 bp[1] End 43,344,529 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 E1 Start 94,411,682 bp[2] End 94,438,136 bp[2]
Gene ontology Molecular function • fibroblast growth factor binding • GO:0001948 protein binding • RNA binding Cellular component • cytoplasm • spliceosomal complex • membrane • cell nucleus • nuclear speck Biological process • negative regulation of fibroblast apoptotic process • negative regulation of apoptotic process • apoptotic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	8539	11800
Ensembl	ENSG00000166181	ENSMUSG00000027193
UniProt	Q9BZZ5	O35841
RefSeq (mRNA)	NM_001142930 NM_001142931 NM_001243747 NM_006595	NM_007466 NM_001305258 NM_001349003
RefSeq (protein)	NP_001136402 NP_001136403 NP_001230676 NP_006586	NP_001292187 NP_031492 NP_001335932
Location (UCSC)	Chr 11: 43.31 – 43.34 Mb	Chr 2: 94.41 – 94.44 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Crystal structure of API5 exhibited the function for protein-protein interaction [7]

Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.

API5 functions in nuclear export of mRNA [8].

References

1. GRCh38: Ensembl release 89: ENSG00000166181 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027193 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Tewari M, Yu M, Ross B, Dean C, Giordano A, Rubin R (September 1997). "AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal". Cancer Research. 57 (18): 4063–9. PMID 9307294.
6. "Entrez Gene: API5 apoptosis inhibitor 5".
7. Han BG, Kim KH, Lee SJ, Jeong KC, Cho JW, Noh KH, Kim TW, Kim SJ, Yoon HJ, Suh SW, Lee S, Lee BI (March 2012). "Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules". The Journal of Biological Chemistry. 287 (14): 10727–37. doi:10.1074/jbc.M111.317594. PMC 3322819. PMID 22334682.
8. Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI (2020). "Regulation of mRNA Export Through API5 and Nuclear FGF2 Interaction". Nucleic Acids Res. 48: 6340–6352. doi:10.1093/nar/gkaa335. PMC 7293033. PMID 32383752.

Further reading

• Gianfrancesco F, Esposito T, Ciccodicola A, D'Esposito M, Mazzarella R, D'Urso M, Forabosco A (1999). "Molecular cloning and fine mapping of API5L1, a novel human gene strongly related to an antiapoptotic gene". Cytogenetics and Cell Genetics. 84 (3–4): 164–6. doi:10.1159/000015247. PMID 10393420.
• Kim JW, Cho HS, Kim JH, Hur SY, Kim TE, Lee JM, Kim IK, Namkoong SE (April 2000). "AAC-11 overexpression induces invasion and protects cervical cancer cells from apoptosis". Laboratory Investigation; A Journal of Technical Methods and Pathology. 80 (4): 587–94. doi:10.1038/labinvest.3780008. PMID 10780674.
• Van den Berghe L, Laurell H, Huez I, Zanibellato C, Prats H, Bugler B (November 2000). "FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2". Molecular Endocrinology. 14 (11): 1709–24. doi:10.1210/me.14.11.1709. PMID 11075807.
• Sutherland HG, Mumford GK, Newton K, Ford LV, Farrall R, Dellaire G, Cáceres JF, Bickmore WA (September 2001). "Large-scale identification of mammalian proteins localized to nuclear sub-compartments". Human Molecular Genetics. 10 (18): 1995–2011. doi:10.1093/hmg/10.18.1995. PMID 11555636.
• Li Z, Hu CY, Mo BQ, Xu JD, Zhao Y (April 2003). "[Effect of beta-carotene on gene expression of breast cancer cells]". Ai Zheng = Aizheng = Chinese Journal of Cancer. 22 (4): 380–4. PMID 12703993.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413.
• Kim JE, Tannenbaum SR, White FM (2005). "Global phosphoproteome of HT-29 human colon adenocarcinoma cells". Journal of Proteome Research. 4 (4): 1339–46. doi:10.1021/pr050048h. PMID 16083285.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Morris EJ, Michaud WA, Ji JY, Moon NS, Rocco JW, Dyson NJ (November 2006). "Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo". PLoS Genetics. 2 (11): e196. doi:10.1371/journal.pgen.0020196. PMC 1636698. PMID 17112319.
• Han BG, Kim KH, Lee SJ, Jeong KC, Cho JW, Noh KH, Kim TW, Kim SJ, Yoon HJ, Suh SW, Lee S, Lee BI (2012). "Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules". J Biol Chem. 287: 10727–37. doi:10.1074/jbc.M111.317594. PMC 3322819. PMID 22334682.
• Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI (2020). "Regulation of mRNA Export Through API5 and Nuclear FGF2 Interaction". Nucleic Acids Res. 48: 6340–6352. doi:10.1093/nar/gkaa335. PMC 7293033. PMID 32383752.