AKAP8

A-kinase anchor protein 8 is an enzyme that, in humans, is encoded by the AKAP8 gene.[5][6]

AKAP8
Identifiers
Aliases	AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95, A-kinase anchoring protein 8
External IDs	OMIM: 604692 MGI: 1928488 HomoloGene: 4278 GeneCards: AKAP8
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19p13.12 Start 15,353,385 bp[1] End 15,379,798 bp[1]
Gene location (Mouse) Chr. Chromosome 17 (mouse)[2] Band 17|17 B1 Start 32,303,672 bp[2] End 32,321,238 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • metal ion binding • DNA binding • zinc ion binding • double-stranded DNA binding • protein kinase A regulatory subunit binding • histone deacetylase binding • DEAD/H-box RNA helicase binding • GO:0001948 protein binding • NF-kappaB binding • RNA binding Cellular component • Golgi apparatus • female pronucleus • membrane • condensed chromosome • cell nucleus • mitochondrion • nucleoplasm • nucleolus • nuclear matrix • cytoplasm Biological process • positive regulation of histone deacetylation • mitotic chromosome condensation • regulation of histone phosphorylation • signal transduction • cell cycle G2/M phase transition • regulation of transcription, DNA-templated • immune system process • cellular response to lipopolysaccharide • cellular response to prostaglandin E stimulus • transcription, DNA-templated • protein transport • negative regulation of tumor necrosis factor production • innate immune system • GO:0007067 mitotic cell cycle • transport Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10270	56399
Ensembl	ENSG00000105127	ENSMUSG00000024045
UniProt	O43823	Q9DBR0
RefSeq (mRNA)	NM_005858	NM_019774 NM_001357760
RefSeq (protein)	NP_005849	NP_062748 NP_001344689
Location (UCSC)	Chr 19: 15.35 – 15.38 Mb	Chr 17: 32.3 – 32.32 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining it to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is redistributed to distinct locations during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.[6]

Interactions

AKAP8 has been demonstrated to interact with:

• Cyclin D3[7]
• DDX5,[8]
• MCM2,[9]
• MYCBP,[10] and
• PRKAR2A.[5][11]

References

1. GRCh38: Ensembl release 89: ENSG00000105127 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024045 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Eide T, Coghlan V, Orstavik S, Holsve C, Solberg R, Skalhegg BS, Lamb NJ, Langeberg L, Fernandez A, Scott JD, Jahnsen T, Tasken K (Mar 1998). "Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95". Exp Cell Res. 238 (2): 305–16. doi:10.1006/excr.1997.3855. PMID 9473338.
6. "Entrez Gene: AKAP8 A kinase (PRKA) anchor protein 8".
7. Arsenijevic T, Degraef C, Dumont JE, Roger PP, Pirson I (Mar 2004). "A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95". Biochem. J. 378 (Pt 2): 673–9. doi:10.1042/BJ20031765. PMC 1223988. PMID 14641107.
8. Akileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM (May 2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi:10.1074/jbc.M101171200. PMID 11279182.
9. Eide T, Taskén KA, Carlson C, Williams G, Jahnsen T, Taskén K, Collas P (Jul 2003). "Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication". J. Biol. Chem. 278 (29): 26750–6. doi:10.1074/jbc.M300765200. PMID 12740381.
10. Furusawa M, Taira T, Iguchi-Ariga SM, Ariga H (Dec 2002). "AMY-1 interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex". J. Biol. Chem. 277 (52): 50885–92. doi:10.1074/jbc.M206387200. PMID 12414807.
11. Collas P, Le Guellec K, Taskén K (Dec 1999). "The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis". J. Cell Biol. 147 (6): 1167–80. doi:10.1083/jcb.147.6.1167. PMC 2168084. PMID 10601332.

Further reading

• Lester LB, Scott JD (1997). "Anchoring and scaffold proteins for kinases and phosphatases". Recent Prog. Horm. Res. 52: 409–29, discussion 429–30. PMID 9238861.
• Michel JJ, Scott JD (2002). "AKAP mediated signal transduction". Annu. Rev. Pharmacol. Toxicol. 42: 235–57. doi:10.1146/annurev.pharmtox.42.083101.135801. PMID 11807172.
• Collas P, Le Guellec K, Taskén K (2000). "The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis". J. Cell Biol. 147 (6): 1167–80. doi:10.1083/jcb.147.6.1167. PMC 2168084. PMID 10601332.
• Steen RL, Cubizolles F, Le Guellec K, Collas P (2000). "A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract". J. Cell Biol. 149 (3): 531–6. doi:10.1083/jcb.149.3.531. PMC 2174845. PMID 10791967.
• Akileswaran L, Taraska JW, Sayer JA, et al. (2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi:10.1074/jbc.M101171200. PMID 11279182.
• Eide T, Carlson C, Taskén KA, et al. (2002). "Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting". EMBO Rep. 3 (5): 426–32. doi:10.1093/embo-reports/kvf089. PMC 1084102. PMID 11964380.
• Furusawa M, Taira T, Iguchi-Ariga SM, Ariga H (2003). "AMY-1 interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex". J. Biol. Chem. 277 (52): 50885–92. doi:10.1074/jbc.M206387200. PMID 12414807.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Eide T, Taskén KA, Carlson C, et al. (2003). "Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication". J. Biol. Chem. 278 (29): 26750–6. doi:10.1074/jbc.M300765200. PMID 12740381.
• Arsenijevic T, Degraef C, Dumont JE, et al. (2004). "A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95". Biochem. J. 378 (Pt 2): 673–9. doi:10.1042/BJ20031765. PMC 1223988. PMID 14641107.
• Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
• Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529–35. doi:10.1038/nature02399. PMID 15057824.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Kamada S, Kikkawa U, Tsujimoto Y, Hunter T (2005). "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association". Mol. Cell. Biol. 25 (21): 9469–77. doi:10.1128/MCB.25.21.9469-9477.2005. PMC 1265837. PMID 16227597.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.

External links

• AKAP8 human gene location in the UCSC Genome Browser.
• AKAP8 human gene details in the UCSC Genome Browser.